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A. Biotin probe-isolated template

WT. 1:1000. A. Biotin probe-isolated template. B. Genomic DNA template.

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A. Biotin probe-isolated template

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  1. WT 1:1000 A. Biotin probe-isolated template B. Genomic DNA template Supplementary Fig. 3. Sequencing of biotin-purified template vs. sequencing of non-purified template, following PNA-PCR. PNA-enriched TaqMan real-time PCR products from purified targets (A) or genomic DNA (B) were subjected to sequencing with forward strand. Duplicate independent experiments are shown. The arrows indicate the position of T790M C>T mutation. When the PNA-PCR amplification is performed directly from genomic DNA (Panel B), mutations emerge even for wild-type DNA. Target purification via biotinylated probe avoids such false-positive mutations (PCR errors) compared with non-target enriched genomic DNA (Panel A). Supplementary Fig. 3

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