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p300. hs. 0 1 2 3 0 1 2 3. Flag-Rev-erb a. HA-p300. Tubulin. Supplemental figure 1: 293T cells were transfected with Flag-Rev-erb a in the presence or absence of p300. Cells were treated

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  1. p300 hs 0 1 2 3 0 1 2 3 Flag-Rev-erba HA-p300 Tubulin Supplemental figure 1: 293T cells were transfected with Flag-Rev-erba in the presence or absence of p300. Cells were treated with cyclohexamide at time 0 and harvested at the indicated tiems. Levels of Rev-erba, p300 and Tubulin were assesed by Western blot with specific antibodies.

  2. WT K591A K400A - + - + - + p300 HA-Rev-erba HA-p300 Supplemental figure 2: 293T cells were transfected with WT HA-Rev-erba or the indicated single mutants in the presence or absence of p300. The levels of Rev-erba and p300 were assesed by Western blot.

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