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Is your mass above 700 Da?

Is your mass above 700 Da?. N. Y. Y. GCEI. Do you require separation of a mixture?. Y. Is it a polymer?. Y. Does it have limited functionality?. MALDI. N. EI. N. N. N. Does it contain only one or two polar functional groups?. Y. EI or CI.

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Is your mass above 700 Da?

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  1. Is your mass above 700 Da? N Y Y GCEI Do you require separation of a mixture? Y Is it a polymer? Y Does it have limited functionality? MALDI N EI N N N Does it contain only one or two polar functional groups? Y EI or CI Is it soluble and contain a number of polar groups? N Y Y Y LSIMS or DART Is it Insoluble? Is it Cationic/Anionic? Do you require separation of a mixture? Is it a peptide or protein? N N N Does it contain a large number of polar groups? Y ES Y Are there buffers or detergents present? N Y ES Will it elute under normal reverse phase conditions? Y N MALDI Y LCES UPC2 Please note: Flow Chart is not exhaustive and should be used as a guide only.

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