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Animal Science/Dairy Science 434. Reproductive Physiology Lecture 1: Introduction, Female Anatomy. Instructor. John J. Parrish 714 Animal Science parrish@calshp.cals.wisc.edu 263-4324. Laboratories. Tuesday, Wednesday or Thursday Room 134 Animal Science 3:30 - 5:30 may last until 6:00.
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Animal Science/Dairy Science 434 Reproductive Physiology Lecture 1: Introduction, Female Anatomy
Instructor • John J. Parrish • 714 Animal Science • parrish@calshp.cals.wisc.edu • 263-4324
Laboratories • Tuesday, Wednesday or Thursday • Room 134 Animal Science • 3:30 - 5:30 • may last until 6:00
Text • Pathways to Pregnancy and Parturition • P.L. Senger • UW Bookstore • Library
Web Page http://www.wisc.edu/ansci_repro/ • Syllabus • Lecture Outlines and Handouts • Labs • Old Exams • Project Information • Important Dates • Grading
434 Web Site Modifications • Updates may occur daily. • Lecture outlines and labs are currently for last year • 2001 lecture updates may be up the night before lecture but I only guarantee them by 8:00 AM the day of lecture. • 2001 lab material will be updated by the time of Tuesday’s lecture.
Web Page Techniques • Netscape 4 or better • Internet Explorer 4 or better • Will work best from a campus networked computer • essential for video clips in WebCT • Display set for 800 X 600 • Frames were used in page construction • Printing (check FAQ) • movement within web site • Reload button • the site is continually being changed
Initial Syllabus Lectures Labs Handouts Old Exams Project FAQ
Important Dates • Lecture Exams • February 22 • April 10 • May 12 (7:25 PM, Sat. ) • Lab Exams • February 27 (given 3X on Tuesday) • May 1 - 3 (taken in lab) • Oral Final • April 16 to end of class
Grading The final grade will be curved but generally ends near the following percentages: A (100-93%), AB (88-93%), B (80-88%), BC (79-70%), C (69-60%), D (59-50%), F (<50%).
Instructions • Graded exercise (5 points) • Write on a sheet of paper what is happening in the video. (2 minutes) • Print your name on the paper • Form groups of 4 or 5; elect recorder and reporter. • Take 4 minutes and discuss what is happening in the video and then make a list of these things. • Print names of group members at bottom of list.
Lecture 1 Female Reproductive Tract Anatomy
A cow is a bull’s way of making another bull. Rex M. Butterfield; The Angus Society of Australia
Learning Objectives • To learn the different structures and nomenclatures for the parts of the female reproductive system. • To learn the basic functions for the different parts of the tract and how they work together.
Names Species Common Adult Adult Name Female Male Bovine cattle cow bull Ovine sheep ewe ram Equine horse mare stallion Procine swine(pig) sow boar
Female Reproductive Tract Sow Uterine Horn Vulva Vagina Broad Ligament Cervix Uterine Body Bladder Oviduct Ovary
Uterine Body Uterine Horn Uterine Horn Ovary Ovary Oviduct Oviduct Cervix Bladder Vagina Urethra Clitoris Vulva
Rectum Uterus Vulva Ovary Vagina Pelvis Bladder Cervix Oviduct Broad Ligament
Broad Ligament • connective tissue sheet which supports and suspends the reproductive tract
Broad Ligament mesometrium mesovarium mesosalpinx
Ovary - Primary Sex Organ • Functions • Hormonal • Estrogen • Progesterone • Female gamete • Oocyte
Follicle Corpus Luteum Corpus Luteum Progesterone Progesterone Ovary - Estrogen, Oocyte
Germinal Epithelium Cortex Medulla Tunica Albuginea Blood and lymphatic Vessels & nerves } Hilus Ovary
Ruptured follicle (ovulation) Tertiary follicle Primary follicle Egg nest Tertiary follicle Secondary follicle Corpus hemorrhagicum Corpus albicans Corpus luteum Atretic follicle
Primordial Follicle Primary Follicle Secondary follicle Tertiary follicle Follicle Cells Nucleus • Flattened cells surround oocyte • Increase in size of oocyte andheight of follicular cells Ooplasma Follicular Cells • Surrounded by severallayers of follicular cells Oocyte • Follicle forms a fluid filled cavity • Differentiation of cell layersin the follicle wall Fluid Cavity Antrum
Follicular Histology Primordial Primary
Follicular Histology Secondary
Tertiary Follicle Theca Externa Theca Interna Basement Membrane Granulosa Oocyte Cumulus Oophorus Antrum (Follicular Fluid)
Tertiary Follicle Primary Follicles Secondary Follicle Theca Interna Granulosa Cumulus Oophorus Oocyte Antrum
Follicular Blood Supply Theca interna cells Basement Membrane Granulosa cells Capillary network Follicular fluid
Secondary Sex Organ: A Series of Tubes to Convey the Oocyte/Embryo/Fetus to the Outside Serosa Submucosa Longitudinal smooth muscle layer Lumen Circular smooth muscle layer Mucosa Muscularis
Functions of Oviduct • Gamete Transport • Sperm • Oocyte • Supports early embryo development • Transport of embryo to uterus
Isthmus Utero-Tubal Junction Ampullary-Isthmic Junction Ampulla Infundibulum Ostium Fimbria Uterine Horn Oviduct Cumulus Oophorus and Oocyte Ovary Follicle
Oviduct Ampulla Mesosalpinx Isthmus UT Junction Uterine Horn
Oviduct Histology Mucosa • Ciliated Columnar • Nonciliated Columnar
Functions of the Uterus • Muscle contractions • Transport sperm • Expel fetus and placenta • Absorption and phagocytosis • Partially prepares sperm for fertilization • capacitation • Provides environment for embryo and fetal growth • Hormone production • Prostaglandin F2a production
Uterus (bovine) horn • Bicornuate • poorly to moderately developed uterine horns • uterine body • one cervix body cervix
Uterine Histology Endometrium Endometrial Glands Perimetrium Myometrium
Uterine Histology Mucosa Endometrial Gland Endometrium Submucosa Myometrium Perimetrium
Functions of the Cervix • Transport of sperm • Barrier to sperm (species dependent) • Reservoir for sperm • Blocking bacterial invasion during pregnancy • Birth canal
Internal Cervical Os External Cervical Os COW Uterine Body • Cervix is composed of thick connective tissue • Mucus is secreted near the time of breeding and ovulation. Cervix Cow has 4-5 annular rings Vagina
Cervix COW Internal Os Cervical Ring Cervical Ring Fornix Fornix External Os Fornix Anterior Vagina EWE Anterior Vagina
Functions of the Vagina • Copulatory organ • Glands secrete lubrication • Birth canal • Glands secrete pheremones
Posterior Vagina (Vestibule) Fornix Vulva-Vaginal Sphincter Mucosa Vagina Anterior Vagina Cervix Stratified Squamous Epithelium Columnar Epithelium Urethra Submucosa Submucosa
Posterior Vaginal Histology Stratified Squamous Epithelium Mucosa Submucosa
Function of External Genitalia • Labia • Closes entrance to vagina • Clitoris • Female sensory organ