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Urine Sample Collection and Laboratory Techniques

Learn the proper techniques for collecting and testing urine samples for various chemical and microscopic examinations. Also, discover common tests done on urine and the collection methods for stool and sputum specimens.

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Urine Sample Collection and Laboratory Techniques

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  1. AL-Mustansiriyah UniversityCollege of scienceBiology Dept.Zoology 4th class Laboratory Technique LAB. (4) NAME :

  2. Urine Sample Urine sample collection rules: • Random. • Clean. • make notes for personal health ( male, female) or taking drugs. • Tested for: • Chemical exam • Specific gravity • pH • Albumin • Glucose • Microscopic exam Directorate of Laboratory Medicine

  3. Directorate of Laboratory Medicine

  4. Common tests done on urine • UA = urinalysis • Urine Culture 1.Urinalysis(normal values) • pH (4.8-8.0) average 6.0 • Protein (none) • Glucose (none) • Ketones (none) • Blood (up to 2 RBC’S) • Specific gravity (1.010-1.025) WBC’S (0-4) • Bacteria (none) Casts (none)

  5. 2. Urine for C&S • Culture = ? Bacteria growing • Sensitivity = which antibiotics are effective • Readings after 24; 48; 72 hrs. Midstream Urine Sterile Catheter Specimen (never from bag)

  6. Stool Specimen Analysis of fecal material can detect pathological conditions for ex: tumors, hemorrhage, infection –……..Tests(Pus,Rbc, Ova & Parasites)

  7. Random Collection • 1. Universal precaution • 2. Collect stool in a dry, clean container • uncontaminated with urine or other body secretions, such as menstrual blood • 4. Collect the stool with a clean tongue blade or similar object. • 5. Deliver immediately after collection

  8. Ova and parasites collection • Warm stools are best for detecting ova or parasites. • Do not refrigerate specimen for ova or parasites. • If the stool should be collect in 10 % formalin or PVA • fixative, storage temperature is not critical. • Because of the cyclic life cycle of parasites, three • separate random stool specimens are recommended.

  9. Sputum Specimens • Culture • Cytology • Tuberculosis • Collection: • Teach to cough effectively….. Not Saliva • Keep inside of container sterile • Document: color, consistency, odor • Early morning specimen

  10. Directorate of Laboratory Medicine

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