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Oxidative phosphorylation Biochemistry, 4 th edition, RH Garrett & CM Grisham, Brooks/Cole ( Cengage ); Boston, MA: 2010 pp 592-629 Instructor: Kirill Popov. The mitochondrion Electron transport Oxidative phosphorylation Heat, oxidative stress, etc .
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Oxidative phosphorylation Biochemistry, 4th edition, RH Garrett & CM Grisham, Brooks/Cole (Cengage); Boston, MA: 2010 pp 592-629 Instructor: Kirill Popov
The mitochondrion Electron transport Oxidative phosphorylation Heat, oxidative stress, etc.
*Numbers of subunits in the bacterial equivalents in parentheses. #Cytochromec is not part of an enzyme complex; it moves between Complexes III and IV as a freely soluble protein.
The P/O ratio is an index of the efficiency of coupling P/O ratio: number of molecules of Pi incorporated (=ATP synthesized) per atom of oxygen consumed (or pair of electrons being carried through the chain). Measurements: oxygen consumption during complete phosphorylation of a fixed amount of ADP after addition of either an NAD+-linked substrate or FAD-linked substrate. P/O 2.5 for NADH P/O 1.5 for FADH2
*The number depends on which shuttle system transfers reducing equivalents into the mitochondrion.