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Into the tiny world…..

Into the tiny world…. Lenses first used to look at things far away Anton Von Leeuwenhoek credited as first person to use lenses to look at things close up (and very small) Two types of light microscope  Simple and compound.

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Into the tiny world…..

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  1. Into the tiny world….. • Lenses first used to look at things far away • Anton Von Leeuwenhoek credited as first person to use lenses to look at things close up (and very small) • Two types of light microscope Simple and compound. • Both work by refracting (bending) light after it passes through a specimen

  2. The Simple Microscope • Single lens • Low magnification • Still very handy

  3. The Compound Microscope • Two lenses • Higher magnification and resolution (more on these next class.) • Revolutionized biology!!!!

  4. The Compound Microscope Ocular lens Course adjustment (focus) Body tube Fine adjustment (focus) nosepiece arm Hi-power objective lens 40 Lo-power objective lens Stage clips 10X stage (Pivot point) Diaphragm base Light source

  5. How to focus • Turn the nosepiece until the lo-power or scanning objective is in place. • Turn coarse adjustment until the stage is as close to the slide as it will go. (it should stop before hitting the objective lens.) • Move the slide until light shines through the sample or region of the slide where you’ll probably find the specimen. • While looking through the ocular lens, slowly turn the coarse adjustment until something comes into focus. (It may be dirt on the top of the coverslip so just move the slide back and forth and see if the image moves.) • Let the hunt begin!

  6. The Crucial step • Once you get your specimen in focus under lo-power, move the slide until it is in the middle of the field-of-view. • WITHOUT CHANGING THE FOCUS, switch to hi-power. • Yes, it will come veeery close to the slide but unless it is a really thick specimen, it shouldn’t touch it.

  7. Safety notables • Carry with two hands. Don’t swing the ‘scope around. • Mind the cord. • NEVER USE THE COARSE ADJUSTMENT WITH HI-POWER OBJECTIVES! (Clear?) • Always take the slide off of the stage, carefully wrap the cord, bag and return microscope to closet when done.

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