ShMOMT3

1. TITLE: Characterization of a flavonol 3-O-methyltransferase in the trichomes of the wild tomato species Solanum habrochaites JOURNAL: Planta AUTHORS: Adam Schmidt, Chao Li, A. Daniel Jones, Eran Pichersky2 2To whom correspondence should be addressed. E-mail: lelx@umich.edu; telephone (734) 936-3522; fax 1 734 647-0884

2. 7 1 b a 318.2 100 ShMOMT3 ShMOMT3 6 Rel. Int. (%) 50 5 [-CH3] 301.2 290.2 333.2 153.2 244.2 219.2 272.2 10 2 4 myricetin 140 160 180 200 220 240 260 280 300 320 340 Relative abundance (TIC) m/z, amu 1 3 318.2 SlMOMT3 SlMOMT3 100 2 2 50 Rel. Int. (%) [-CH3] 1 333.2 290.2 244.2 153.2 219.2 10 myricetin 0 140 160 180 200 220 240 260 280 300 320 340 5 6 7 8 9 10 Retention time (min) m/z, amu c M1-3′ M Q1-3 Q origin A B C D A B C D ShMOMT3 SlMOMT3 Suppl. Fig. S1 LC/MS and radio-TLC analyses of products formed in reactions catalyzed by ShMOMT3 or SlMOMT3 with myricetin (LC/MS) and other flavonol substrates (radio-TLC). a, Products analysis of the reaction with myricetin. Peak 1 is unreacted myricetin, peak 2 is 3-O-methyl myricetin. b, Mass fragmentation patterns of peak 2 in chromatograms (a). c, Products formed from reaction of ShMOMT3 or SlMOMT3 with substrates (A) myricetin, (B) 3′-O-methyl myricetin, (C) quercetin, (D) 3-O-methyl quercetin. Authentic standards: (Q) quercetin, (Q1-3) 3-O-methyl quercetin, (M) myricetin, (M1-3′) 3′-O-methyl myricetin

3. a b Velocity (umol s-1) Velocity (umol s-1) c d Velocity (umol s-1) Velocity (umol s-1) Suppl. Fig. S2 Plots of velocity measurements from reaction of recombinant ShMOMT3 with a, myricetin; b, 3′-O-methyl myricetin; c, 7-O-methyl quercetin; d, [Methyl-14C] SAM