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REPLICATION

REPLICATION. DEF: WHEN DNA MAKES COPIES IF ITSELF. PARTS:. A) HELICASE—IT’S AN ???? JOB: UNWINDS DNA STRANDS AND KEEPS THEM SEPARATED B) DNA POLYMERASE: LINKS NUCLEOTIDES TO GROWING STRAND. Helicase. DNA Polymerase. DNA LIGASE :.

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REPLICATION

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  1. REPLICATION DEF: WHEN DNA MAKES COPIES IF ITSELF

  2. PARTS: • A) HELICASE—IT’S AN ???? JOB: UNWINDS DNA STRANDS AND KEEPS THEM SEPARATED • B) DNA POLYMERASE: LINKS NUCLEOTIDES TO GROWING STRAND

  3. Helicase

  4. DNA Polymerase

  5. DNA LIGASE: ENZYME THAT LINKS THE OKAZAKI FRAGMENTS TOGETHER ON THE LAGGING STRAND DURING REPLICATION

  6. OKAZAKI FRAGMENTS PIECES OF THE LAGGING STRAND

  7. Okazaki fragments

  8. The Okazaki’s Reiji Okazaki (husband) Tsuneko Okazaki (wife) (discovered THE fragments in 1968) Okazaki died of leukemia seven years after his discovery; he had been heavily irradiated in Hiroshima when the first atomic bomb was dropped.

  9. PRIMASE • ENZYME THAT MAKES A DNA PRIMER

  10. primase

  11. PRIMER • STARTER • SHORT RNA SEGMENT THAT ISCOMPLIMENTARY TO DNA STRAND; GIVES NEW DNA PLACE TO ATTACH

  12. REPLICATION FORK • WHERE DNA SPLITS—ACTIVE REPLICATION IS OCCURRING

  13. LEADING STRAND • STRAND ON PARENT MOLECULE THAT RUNS 5’ TO 3’; CONTINUOUS REPLICATION IS HAPPENING—NO OKAZAKI FRAGMENTS

  14. LAGGING STRAND • PARENT DNA STRAND THAT RUNS 3’ TO 5’ …REPLICATION IS NOT CONTINUOUS

  15. REPLICATION OCCURS… • 5’ TO 3’

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