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7-AAD

Aphidicolin. Untreated. BrdU + : 74% + 2 S/G2/M: 69% + 2. BrdU + : 3% + 0.2 S/G2/M: 47% + 1. Wt Ctrl. BrdU + : 76% + 5 S/G2/M: 73% + 4. BrdU + : 3% + 2 S/G2/M: 47% + 1. Wt Cre. BrdU + : 72% + 8 S/G2/M: 68% + 1. BrdU + : 3% + 0.4 S/G2/M: 46% + 3.

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7-AAD

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  1. Aphidicolin Untreated BrdU+: 74% + 2 S/G2/M: 69% + 2 BrdU+: 3% + 0.2 S/G2/M: 47% + 1 Wt Ctrl BrdU+: 76% + 5 S/G2/M: 73% + 4 BrdU+: 3% + 2 S/G2/M: 47% + 1 Wt Cre BrdU+: 72% + 8 S/G2/M: 68% + 1 BrdU+: 3% + 0.4 S/G2/M: 46% + 3 Atrflox Ctrl BrdU+: 1.2% + 0.1 S/G2/M: 47% + 1 BrdU+: 71% + 5 S/G2/M: 66% + 3 Atrflox Cre BRDU 7-AAD Supplementary Figure S3.Cell cycle status of ATR-deleted fibroblasts. Subconfluent fibroblast cultures from Atrflox/- mice or wildtype B6 mice were transduced with MSCV-IRES-GFP or MSCV-Cre-IRES-GFP. Cells were cultured for 4 days, splitting as necessary, and replated for 1 day before treatment with 4 M aphidicolin. Before treatment and 16 hours after addition of aphidicolin cells were labeled with 10 M BrdU for 75 minutes and examined for BrdU incorporation and DNA content as described (Tough, D. F. & Sprent, J J. Exp. Med.179, 1127–1135 (1994)). Raulet et al (MS 2004-12-27903B-Z)

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