C-myc paper reviewer’s suggestions

1. C-myc paper reviewer’s suggestions • Use additional SERCA inhibitor to show affects on c-myc, egr-1 and MAPKs • Look at c-myc and egr-1 at the protein level when induced with Tg. • Use U73343 which is the less active analog of the PLC inhibitor U73122 to control for non-specific effects

2. What now • Treat Perk+/+ and Perk-/- MEFs with cyclopiazonic acid (CPA) • Look at eIF2a phosphorylation • Assess c-myc and egr-a at RNA and protein level • Treat Perk+/+ and Perk-/- MEF with Tg • Look at c-myc and egr-1 at protein level

3. UT CPA hr CPA 2hr Tg UT CPA hr CPA 2hr Tg WT KO WT KO WT KO WT WT KO WT KO WT KO WT eIF2a[P] eIF2a Perk+/+ and Perk-/- MEFs treated with 10mM CPA

4. Image Quant