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National Prevalence of Transmitted HIV Drug Resistance in Swaziland in 2011

National Prevalence of Transmitted HIV Drug Resistance in Swaziland in 2011. R. Suzanne Beard, Ph.D. Abstract/poster : TUPDC0103. Background.

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National Prevalence of Transmitted HIV Drug Resistance in Swaziland in 2011

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  1. National Prevalence of Transmitted HIV Drug Resistance in Swaziland in 2011 R. Suzanne Beard, Ph.D. Abstract/poster: TUPDC0103

  2. Background • Assessed the national level of transmitted drug resistance (TDR) in recently HIV-infected adults (18-49 yrs.) using samples collected from seroconverters and acutely HIV-infected persons enrolled between 2010-2011 in the Swaziland HIV Incidence Measurement Survey (SHIMS) • 18,172 adults completed household-based counseling and rapid HIV testing • 13 seronegative, NAT positive(acute) • 11,232 HIV-negative individuals had repeat HIV testing six months later, 145 seroconverted (SC) • 151 out of 158 HIV-positive samples were available for HIVDR analysis Abstract/poster: TUPDC0103

  3. Resistance Profile by Assay for Patients With TDR • Genotyping was performed with 3assays: sequencing based (in-house), multiplex allele-specific (MAS), and allele-specific real-time PCR (AS-PCR) • detection limits of minor mutations of 20%, 1.6-12.5% and 0.2-1.7% respectively • TDR mutations were determined by Calibrated Population Resistance tool using the 2009 WHO DR mutation list Abstract/poster: TUPDC0103

  4. Conclusions These findings provide the first estimate of the national prevalence of TDR, using samples from a nationally representative cohort of HIV seroconverters and acutely HIV-infected individuals Using WHO definitions, the TDR level in Swaziland is: low (<5%) based on the sequencing-based in-house or more sensitive MAS assay moderate (5-15%) based on the ultrasensitive AS-PCR Further work needs to be done to determine the clinical significance of low-frequency DR mutations Surveillance of TDR in recently HIV-1-infected populations can inform treatment programs on the efficacy of current ART regimens available Center for Global Health Division of Global HIV/AIDS Abstract/poster: TUPDC0103

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