nyc1 / nol

1. A B 0 35 0 54 0 35 0 54 WT nyc1/nol WT nyc1/nol Months of storage Months of storage C D kD TAG 250 150 100 75 50 37 Oxidized TAG 25 20 15 M 0 35 0 54 nyc1/nol WT 0 35 0 54 Months of storage nyc1/nol WT Months of storage Supplemental Figure S2. Protein and lipid contents in WT seeds and nyc1/nol mutant seeds (fresh and old seeds). A, Protein contents of seeds. Total protein was extracted from 10 dry seeds and quantified. The error bars represent the S. D. of three samples. B, Lipid contents of seeds. Total lipid was extracted from 10 to 50 mg of seeds and quantified. The error bars represent the S. D. of three samples. C, SDS-PAGE analysis of total protein of seeds. Total protein was extracted from 50 seeds with 200 μL buffer, and 10 μL of the extract was used for the analysis of SDS-PAGE. Gel was stained with CBB. D, TLC analysis of total lipid of seeds. Total lipid extracted as described in (B) was separated on a silica TLC plate developed with hexane:diethylether:acetic acid (60:40:1, v/v). Lipids were visualized by exposure to sulfuric acid.