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EMBOSS GUI. 2k81217. EMBOSS. http://bioinfo.nhri.org.tw/. EMBOSS. Command Mode EMBOSS - functions complete - establish personal database - Unix interface JEMBOSS - HTML interface, personal account required - functions limited - java windows EMBOSS GUI
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EMBOSS GUI 2k81217
EMBOSS http://bioinfo.nhri.org.tw/
EMBOSS • Command Mode EMBOSS - functions complete - establish personal database - Unix interface • JEMBOSS - HTML interface, personal account required - functions limited - java windows • EMBOSS GUI - concise, easy to use - Web browser, no account needed
http://bioinfo.nhri.org.tw/gui/ EMBOSS GUI
http://bioinfo.nhri.org.tw/gui/ EMBOSS GUI
Additional web sites of EMBOSS http://bioweb.pasteur.fr/seqanal/EMBOSS/ http://csc-fserve.hh.med.ic.ac.uk/emboss.html
Tools in EMBOSS • Sequence analysis: RE MAP, ORF, translation, GC content REMAP, GETORF, PLOTORF, GEECEE • Sequence alignment: NEEDLE, WATER, EMMA, SHOWALIGN, PRETTYPLAT • Scans DNA sequences for transcription factors: TFSCAN • Function prediction: Motif and Profiles PATMATMOTIFS • Protein 2D structure: HELIXTURNHELIX TMAP
Restriction Enzyme MAP REMAP
Restriction cutting sites translation
GETORF Finds and extracts open reading frames (ORFs)
GETORF Finds and extracts open reading frames (ORFs)
PLOTORF Plot potential open reading frames
GEECEE Calculates fractional GC content of nucleic acid sequences
GEECEE Calculates fractional GC content of nucleic acid sequences
Sequence alignment • Global alignment: compare the whole similarity and length of two sequence NEEDLE • Local alignment: find a most identical/similarity region of two sequence WATER • multiple sequence alignment: EMMA, SHOWALIGN, PRETTYPLAT
Global alignment NEEDLE Sequence 1 Sequence 2
NEEDLE Global alignment
Local alignment WATER Sequence 1 Sequence 2
Local alignment WATER
Multiple sequence alignment EMMA copy
Displays a multiple sequence alignment SHOWALIGN
Displays a multiple sequence alignment SHOWALIGN Identical sequence
Displays aligned sequences, with colouring and boxing PRETTYPLOT
PRETTYPLOT Displays aligned sequences, with colouring and boxing • PRETTYPLOT
TFSCAN Scans DNA sequences for transcription factors