1 / 17

Molecular detection of haemoparasites in dogs in Mnisi Mpumalanga Province, South Africa

Molecular detection of haemoparasites in dogs in Mnisi Mpumalanga Province, South Africa. A.O. Kolo , K. Sibeko-Matjila, D. Knobel & P.T. Matjila. List of Contents. Introduction Objectives of the Study Materials and Methods Results Discussion/Conclusion Acknowledgment. Introduction.

mahala
Download Presentation

Molecular detection of haemoparasites in dogs in Mnisi Mpumalanga Province, South Africa

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Molecular detection of haemoparasites in dogs in Mnisi Mpumalanga Province, South Africa A.O. Kolo, K. Sibeko-Matjila, D. Knobel & P.T. Matjila

  2. List of Contents • Introduction • Objectives of the Study • Materials and Methods • Results • Discussion/Conclusion • Acknowledgment

  3. Introduction • Canine vector-borne diseases have a worldwide distribution and are increasingly significant as emerging diseases • Transmitted by ticks, fleas, sand flies and mosquitoes

  4. Common haemoparasites of dogs

  5. Objective of the study • Not much data is available on the haemoparasites of dogs in Mnisi • Screen blood samples from domestic dogs in Mnisi area, Bushbuckridge for haemoparasites using the reverse line blot (RLB) hybridisation assay

  6. Materials and Methods • Sample collection- • 141 blood samples collected from domestic dogs and stored on sterile FTA filter cards • DNA extraction- DNA was extracted from FTA cards using QIAamp DNA mini kit®(Qiagen) according to manufacturer’s instructions. Eluted DNA was stored at -20°C until further analysis by PCR

  7. Materials and Methods • PCR amplification

  8. Materials and Methods

  9. Materials and Methods PCR conditions: optimized using a Touchdown PCR

  10. Materials and Methods • Reverse line blot hybridisation assay- was performed on PCR products according to (Gubbels et al, 1999; Matjila et al, 2004) • PCR products were hybridized unto a membrane with specific oligonucleotide probes

  11. Results

  12. Results

  13. Results

  14. Discussion/Conclusion • Ehrlichia canis is the most common haemoparasite present in dogs in Mnisi. • Almost 50% of samples tested positive for the genus-specific probe of Ehrlichia/ Anaplasma. There will be a follow-up sequencing to find out if novel species or variants of existing species are detected • 14 samples (9.9%) had mixed infections which could be related to the distribution of different arthropod vectors being present on the same host at a time

  15. Discussion/Conclusion • This study presents an RLB assay that simultaneously detects and differentiates all major haemoparasites that are present in dogs in Mnisi, Mpumalanga Province • The study provides preliminary data on the occurrence of haemoparasites of domestic dogs in Mnisi • The study buttresses the importance of effective control measures to prevent the transmission of these haemoparasites to domestic dogs in Mnisi, and South Africa as a whole

  16. Acknowledgement • Dr Kgomotso Sibeko-Matjila • Prof Tshepo Matjila • Prof Darryn Knobel • Mrs Milana Troskie • Ms Ilse Vorster

  17. THANK YOU!

More Related