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Summary of Molecular Biology course 2009 Feb-June

Summary of Molecular Biology course 2009 Feb-June. 课程内容 Contents. Molecular Biology of the Gene, 5/6/E --- Watson et al. (2004/2007). Part I: Chemistry and Genetics Part II: Maintenance of the Genome* Part III: Expression of the Genome*** Part IV: Regulation*** Part V: Methods**.

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Summary of Molecular Biology course 2009 Feb-June

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  1. Summary of Molecular Biology course2009 Feb-June

  2. 课程内容Contents

  3. Molecular Biology of the Gene, 5/6/E--- Watson et al. (2004/2007) Part I: Chemistry and Genetics Part II: Maintenance of the Genome* Part III: Expression of the Genome*** Part IV: Regulation*** Part V: Methods**

  4. The revised central dogma ***** 1. 基因组的保持 The structure of DNA and RNA 2. 3. 基因组的表达 4. RNA processing 5-6 Gene regulation 7-9 10. Method

  5. 学时分配和教学日历

  6. 教学理念For future & LOVE

  7. Shaping the future of my students/the world in Wuhan University

  8. We have worked together closely and hard to prepare you to meet today’s challenges and become leaders of the future. www.themegallery.com

  9. 世界是平的，英语是当今的国际语言。你们的英语能力提高了。世界是平的，英语是当今的国际语言。你们的英语能力提高了。 A language means more opportunities 创新是灵魂，你们发现问题和解决问题的能力提高了。 Identify the problem and resolve it in a proper and efficient way. 团队(爱)让人坚固，你们的团队意识增强了。Team work is an enjoyable living style www.themegallery.com

  10. 你们的能力增强了: English skills, Research skills/ Resolving problem, Team work skills 你们的素质提升了: 诚实（integrity） 欣赏和热爱生命（appreciate/love life）， 合作 (cooperation/love others) www.themegallery.com

  11. 教学策略 passion & creativity

  12. 教学策略 因“材”施教：创造教师和学生之间的黄金组合，充分挥洒老师和学生对生命和对分子的热爱。 爱能创造奇迹，Love never fails www.themegallery.com

  13. . 教师团队 鸣谢 郭老师和朱老师，以及许光等六位TAs。 郭德银教授 副院长 珞珈特聘教授 朱应教授 www.themegallery.com

  14. New For 2009 • 小组学习：自由组合的创新突破项目小组。 • 为欣赏生命之精美和神奇，为热爱生命而学习。 2009的创意决定（2月17日发布） www.themegallery.com

  15. 多元化考核 • 平时小考（15％）：养成平时学习的好习惯，共2次【诚信从小考做起】。 • 小组学习项目（25％）：项目申请报告5分，总结报告5分，项目执行情况5分，文献报告10分。 • 期末考试（60％）：知识掌握和综合运用能力。

  16. 期末复习

  17. CHAPTER 6 The structures of DNA and RNA The structure differences between DNA and RNA, and why DNA is suitable to serve as the genetic material and RNA can act as a catalyst and regulator etc (please review the diverse function of RNA molecule) .

  18. CHAPTER 8 The replication of DNA The Chemistry of DNA Synthesis The Mechanism of DNA Polymerase The Specialization of DNA Polymerases The Replication Fork DNA Synthesis at the Replication Fork Initiation of DNA Replication ( 原核&真核) Binding and Unwinding Finishing Replication ( 原核&真核) I. Reaction & Catalyst II. Replication at a fork 前导链与后随链 III. Initiation & Termination

  19. Chapter 12: Mechanisms of Transcription • RNA polymerases (RNAP, 真核和原核的异同) and transcription cycle (Initiation, elongation and termination) • Transcription cycle in bacteria***: • Initiation: (1) The feature of s70 promoters. (2) Promoter binding by s70 transcription factor (recognition mechanism). (3) Transition to open complex. (4) Promoter escape and transition to the ternary complex. • Elongation and editing by polymerase (10-4) • Termination: Rho-independent and Rho-dependent mechanism.

  20. Transcription in eukaryotes by RNAP II***: • -- • -Initiation • (i) Cis-acting elements: core promoter & regulatory sequences • (ii) Formation of the pre-initiation complex • (iii) Promoter escape and the CTD tail • (iv) The function of each GTF (in vitro) • (v) Additional proteins for in vivo transcription. • ---Elongation and proofreading involve a new set of GTFs (What) • ---Coupled with RNA processing (How) • ---5’ capping and 3’ polyadenylation (mechanisms) • ---Termination: an RNase and two models

  21. Chapter 13 • Why RNA splicing is important? • Chemical reaction: determination of the splice sites, the products, trans-splicing • Spliceosome assembly: splicing pathway and finding the splice sites.**** • Self-splicing introns and mechanisms *** • Alternative splicing and regulation, alternative spliceosome **** • Two different mechanisms of RNA editing* • mRNA transport-a link to translation *

  22. Chapter 14-topics Topics 1-4:Four components of translation machinery. T1-mRNA; T2-tRNA; T3-Attachment of amino acids to tRNA (aminoacyl-tRNA synthetases); T4-The ribosome Topic 5-6:Translation process. T5-initiation; T6-elongation; T7-termination. Topic 8:Translation-dependent regulation of mRNA and protein stability

  23. Chapter 14- key points • The structure and function of four components of the translation machinery. (****) • Translation initiation, elongation and termination (具体过程和翻译因子的作用-注意起始阶段原核与真核的不同，****) • Translation-dependent regulation of the stability of defective mRNAs and the resulted protein (生物学问题是什么，在原核和真核分别怎么解决的**)

  24. Chapter 15- key points • What is the degeneracy of genetic code? what is its importance? • What are the three roles governing the genetic code? What are the best known intergenic suppressor genes? • What are the benefits of the code universality?

  25. Chapter 16- key points • Principles of gene regulation.(1) who regulate? (2) where to target? (3) How to regulate? • Regulation of transcription initiation in bacteria:the lac operon, alternative s factors, NtrC, MerR, araBAD operon. • The case of l phage--layers of regulation: l repressor and Cro and their binding; control of the lytic and lysogenic growth; lysogenic induction.

  26. Chapter 17- key points TOPIC 1 Conserved structures of eukaryotic transcription activators (2 separable domains and 2 techniques). TOPIC 2 Recruitment of Protein Complexes to Genes by Eukaryotic Activators. (two classes of complexes ) (2 techniques) TOPIC 3 Transcriptional Repressors (4 ways of repression) TOPIC 4 two examples of signal integration and one example of combinatorial control.

  27. TOPIC 5 Common pathway of signal transduction: and 4 different ways to Control transcriptional Regulators. Two examples (STAT, MAPK) TOPIC 6 Gene Silencing by Modification of Histones and DNA (Yeast telomere, YP1 in Drosaphila, DNA methylation in mammalian, imprinting example HP19-Igf2). TOPIC 7 Epigenetic Gene Regulation: concept, the inheritance of DNA methylation.

  28. Chapter 18 key points • 细菌中RNA调控的三种机制：sRNA, riboswitches, attenuation • RNA干扰在真核基因表达调控中的功能 • （1）RNA干扰的发现和作用机制 • （2）miRNA的发现、生成机制和调控机制。 • （3）miRNA的生物学功能，例子lin-4 • （4）miRNA在癌症发生中的作用研究思路 • （5）siRNA的产生方法及其在科研和治疗上的应用。

  29. Chapter 20- key points 你在本章中所学到的核酸和蛋白质技术的作用原理和主要用途

  30. Nucleic acids techniques: Electrophoresis; Restriction digestion; Hybridization (southern, northern, clony); PCR amplification (regular, RT-PCR, mutational PCR); Sequencing (Sanger), genome sequencing (Shotgun and genome assembly), genomic analysis (protein gene identification in bacteria and animals, BLAST search to predict the function of a new protein) DNA cloning and gene expression. (How to analyze a clone, and how to make a fusion protein)

  31. Protein techniques: Protein purification: column chromatography and affinity chromatography; Protein separation by PAGE and identification by western blot Protein sequencing (Edman degradation and tandem mass spectrometry); Proteomics (combine 2-D gel separation and tandem MS ). Study the interactions: Protein-nucleic acids in vitro:Gel retardation & Dnase I footprinting Protein-DNA in cells: Chromatin IP Protein-protein: yeast two-hybrid, Co-IP Determining the Structure of protein and nucleic acids: X-ray crystallography, NMR

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