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Cardiovascular TE mini-presentations

Cardiovascular TE mini-presentations. Heart at the microscale - Philicia Tissue engineering: scaffolds - Julianne Tissue engineering: cell sources - Suzy Histology overview - Kelsey Immunohistochemistry - Ashley Reminder: Reference EVERYTHING!. Cells in a Heart.

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Cardiovascular TE mini-presentations

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  1. Cardiovascular TE mini-presentations • Heart at the microscale- Philicia • Tissue engineering: scaffolds - Julianne • Tissue engineering: cell sources - Suzy • Histology overview - Kelsey • Immunohistochemistry - Ashley • Reminder: Reference EVERYTHING!

  2. Cells in a Heart • cardiac muscle and connective tissue. • myocytes • complete mixture of: collagen fibrils, elastin, cells including fibroblasts and macrophages, macromolecules such as glycoproteins, and glycosaminoglycans together with other molecules such as growth factors, cytokines, and extracellular proteases http://www.cellsalive.com/myocyte.htm http://en.wikipedia.org/wiki/Heart http://ajpheart.physiology.org/content/289/3/H973.full

  3. Tissue Engineering Scaffolds Replace, restore, regenerate defective tissue Tissue engineering triad Functions and Features: • Architecture • Tissue Compatibility • Bioactivity • Mechanical Property Cells are ‘seeded’ into the scaffold to promote 3D growth Figure 1: Engineered Vascular Graft Figure 2: Difference between porous PEG/PBT and cartilage grafts

  4. Works used/ Good resources http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2587658/ http://en.wikipedia.org/wiki/Tissue_engineering#Scaffolds Images: http://www.nature.com/nmat/journal/v4/n7/fig_tab/nmat1421_F6.html http://en.wikipedia.org/wiki/File:Gef%C3%A4%C3%9Fprothese.JPG

  5. TE Cell Sources • Tissue engineering uses primary cells -> cells from organisms • American Type Culture Collection • NIH • Biotechnology companies • Invitrogen • Cell banks • Can borrow from other labs but risk contamination!

  6. Histology: Tissue Preparation • Fixation– preserve the sample by killing the tissue and bacteria (formalin, Picric acid) • Dehydration – replace water in sample with ethanol • Clearing - replace ethanol with xylene (increase transparency)    • Embedding – replace xylene with paraffin (Infiltration typically occurs in an oven at 58 -60°C) • Sectioning– trim and slice the tissue/paraffin block into thin sections (should make a ribbon of samples in successive order) • Mounting - place onto microscope slides (pretreated with egg albumen adhesive) • Staining  – differentiate between the acid and basic components of the cells or the fibrous components of the extracellular matrix; stain with fluorescent molecule  or radioactive isotope or dye

  7. IHC - Ashley

  8. http://www.piercenet.com/browse.cfm?fldID=F95B91A9-3DC1-4B56-8E8D-59CA044A8BA7http://www.piercenet.com/browse.cfm?fldID=F95B91A9-3DC1-4B56-8E8D-59CA044A8BA7 • http://www.youtube.com/watch?v=mpK5ojrXQs0

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