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WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni. Crops2Industry “Non-food Crops-to-Industry schemes in EU27”. Work Package 2: Plant breeding.
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WP 2Tasks: 2-1 to 2-3Task leader: Dimitra Milioni Crops2Industry “Non-food Crops-to-Industry schemes in EU27”
Work Package 2: Plant breeding Objectives: To identify current molecular genetic technologies (genomic and biotechnological tools) and suggest their potential applications in a crop-specific manner to address a wide range of breeding constraints • Task 2.1: To survey the generic tools currently available for the selected non-food crops/species research, breeding production and processing • Task 2.2: To identify European Genetic Resource Centres • Task 2.3: To capitalize on biotechnology tools and exploit the knowledge of the post genomic era for possible future non-food crops improvement
Spanning the gap between genomics and breeding. Varshney et al.,2010
Work Package 2: Plant breeding Objectives: To identify current molecular genetic technologies (genomic and biotechnological tools) and suggest their potential applications in a crop-specific manner to address a wide range of breeding constraints • Task 2.1: To survey the generic tools currently available for the selected non-food crops/species research, breeding production and processing • Task 2.2: To identify European Genetic Resource Centres • Task 2.3: To capitalize on biotechnology tools and exploit the knowledge of the post genomic era for possible future non-food crops improvement
European Genetic Resource Centres Germplasm as a starting point • European Cooperative Crop Genetic Resources Program (ECPGR) • EURISCO, a web-based catalogue • Centre for Genetic Resources, The Netherlands (CGN) • Centre for Plant Genetic ResourcesPoland, Blonie, Poland • Istituto di Genetica Vegetale (Germplasm Research Institute), Italy • The Nordic Gene Bank, Alnarp, Sweden • The Research Institute of Crop Production, Czech Republic • UK Plant Genetic Resources Group, UK • Germplasm Resources Information Network (GRIN), USA
Molecular Genomics Resource CentresNot one gene but all the genes • European Molecular Biology Laboratory – European Bioinformatics Institute (EMBL-EBI) • National Centre for Biotechnology Information (NCBI) • DNA Databank in Japan (DDBJ) • The Institute for Genome Research (TIGR) • PlantGDB • Brassica Genome Gateway • GRAMENE
Orthologous gene families between sorghum, Arabidopsis, rice and poplar. Whole genome shotgun sequencing project AH Patersonet al.Nature457, 551-556 (2009) doi:10.1038/nature07723
Alignment of sorghum, rice and maize. AH Patersonet al.Nature457, 551-556 (2009) doi:10.1038/nature07723
Molecular markers to study genetic diversity in sogrhum germplasms • Simple Sequence Repeats (SSR) • Inter Simple Sequence Repeats (ISSR) • Random Amplified Polymorphic DNA (RAPD) • Amplified Fragment Length Polymprphism (AFLP) • Diversity Arrays Technology (DArT) • Single Feature Polymorphism (SFP) • Quantitative Trait Locus (QTL)
Genetic mapping of QTLs for sugar-related traits in a RIL population of Sorghum bicolor L. Moench Shiringani et al., TAQ (2010); 121(2):32336.
An Induced Sorghum Mutant Population Section of the leaf blade from BTx623, P5A3, and P7H6 Gallery of selected mutant phenotypes
Genetic transformation of Sorghum Production of transgenic sorghum plants Plant Cell, Tissue and Organ Culture doiI: 10.1007/s11240-007-9228-1 Development of transgenic sorghum for insect resistance against the spotted stem borer (Chilo partellus). TRANSGENIC SWEET SORGHUM WITH ALTEREDLIGNIN COMPOSITION AND PROCESS OF PREPARATION THEREOF (United StatesPatent Application 20100058496)
Thank you for your attention Crops2Industry “Non-food Crops-to-Industry schemes in EU27”