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Sockeye Salmon Captive Broodstock Program: Spawning Review

Sockeye Salmon Captive Broodstock Program: Spawning Review. Program goals. Avoid population extinction using captive technology. Tier 1. Conserve population genetic diversity. Increase the number of individuals in the population.

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Sockeye Salmon Captive Broodstock Program: Spawning Review

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  1. Sockeye Salmon Captive Broodstock Program:Spawning Review

  2. Program goals Avoid population extinction using captive technology. Tier 1 Conserve population genetic diversity. Increase the number of individuals in the population. Move from a captive broodstock to an integrated broodstock and eventually de-list the population Tier 2

  3. Spawning Redfish Lake Sockeye Salmon • Captive broodstock spawning principles: • Equalize family size • Factorial Spawning • Maximize the number of breeders per generation • - Minimize inbreeding (relatedness)

  4. Pre-season….. Genetic samples are processed for the Age-3 captive broodstock.

  5. Pre-season….. Ultrasound all Age-3 captive broodstock to determine maturation status and sex.

  6. Pre-season….. Genotype returning anadromous sockeye to determine which fish to incorporate into the captive broodstock.

  7. Pre-season….. Develop the spawning matrix.

  8. Spawning Redfish Lake Sockeye Salmon Preparation: • Determine which males are running. • Determine which females are ready. • Develop crosses.

  9. Spawning Redfish Lake Sockeye Salmon Female Station: • Strip green eggs into one gallon zip lock • Add oxygen • Place eggs in cooler

  10. Spawning Redfish Lake Sockeye Salmon Male Station: • Identify males needed to cross with female • Collect milt in whirl-pak • Check motility of milt. • Add oxygen • Place in cooler

  11. Spawning Redfish Lake Sockeye Salmon Fertilization Station: • Split green eggs into three equal groups. • Fertilize eggs with milt from appropriate male. • 20 minute iodophor disinfection.

  12. Spawning Redfish Lake Sockeye Salmon Incubation: • Label incubator. • Return to incubation area.

  13. Spawning Redfish Lake Sockeye Salmon The Next Generation: • Equalize Family Size • Select one or two eggs from each spawn cross.

  14. Spawning Redfish Lake Sockeye Salmon Program Challenges: • Spawn Timing – Anadromous vs Captive. • Isolation between Anadromous and Captive stocks. • Low volumes of milt from captive males. • Delay before fertilization occurs. • Small number of sockeye spawned per day. Questions

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