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Adenoviridae

Adenoviridae. Molecular Virology. HISTORY. first isolated in 1953 (Rowe,  et al.  (1953), Proc. Soc. Exp. Biol. Med. 84:570–573) from tonsils and adenoids of children similar viral agents were isolated from febrile military personnel with respiratory illnesses

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Adenoviridae

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  1. Adenoviridae Molecular Virology

  2. HISTORY • first isolated in 1953 (Rowe, et al. (1953), Proc. Soc. Exp. Biol. Med.84:570–573) from tonsils and adenoids of children • similar viral agents were isolated from febrile military personnel with respiratory illnesses • adenoid degeneration (AD), adenoid-pharyngeal conjunctival (APC), and acute respiratory disease (ARD) agents • 1956  ADENOVIRUS passage normal cell culture cytopathic effect virions

  3. TAXONOMY GROUP Group I dsDNA FAMILY Adenoviridae (Greek, adenos, “gland”) GENUS Mastadenovirus (Greek, mastos, “breast”)                         Aviadenovirus (Latin, avis, “bird”)                         Atadenovirus (English, adenine and thymine)                         Siadenovirus (English, sialidase) Ichtadenovirus(Greek, ichthys, “fish”)

  4. TYPE SPECIES Sturgeon Adv A Frog Adv Fowl Adv A Ovine Adv D Human Adv C

  5. CLASSIFICATION SCHEMES FOR HUMAN ADENOVIRUS (MASTADENOVIRUS H) Knipe and Howley. Field’s Virology. 5th ed.

  6. GENOME • linear dsDNA; 30-36 Kb • inverted terminal repeat (ITR) sequences at 3’-end; 30-200 bp • linear DNA forms a loop due to terminal protein (covalently linked to each 5’ end) and terminal base pairing • all genes are transcribed by host RNA pol II except VA gene which is transcribed by RNA pol III • mRNAs are polycistronic and are differentiated by alternative splicing and use of different poly(A) sites

  7. STRUCTURE • non-enveloped, icosahedral capsid; 90-120 nm • 20 equilateral triangular faces, 12 vertices • each face has 12 identical hexons and each vertex has 1 penton; • each penton is attached to a fiber protein • 12 x 20 = 240 hexons; also 12 pentons • total of 252 capsomers

  8. CAPSID Protein II (hexon monomer) - structural - neutralizing Abs directed against the ε epitope - type specific Ag sites

  9. CAPSID Protein II Protein III (penton base) - penetration

  10. CAPSID Protein II Protein III Protein IIIa - penetration

  11. CAPSID Protein II Protein III Protein IIIa Protein IV (fiber protein) - receptor attachment - hemagglutination - type-specific and some species-specific Ag sites

  12. CAPSID Protein II Protein III Protein IIIa Protein IV Protein VI - hexon minor polypeptide - stability/particle assembly

  13. CAPSID Protein II Protein III Protein IIIa Protein IV Protein VI Protein VIII - hexon minor polypeptide - stability/particle assembly

  14. CAPSID Protein II Protein III Protein IIIa Protein IV Protein VI Protein VIII Protein IX - hexon minor polypeptide - stability/particle assembly

  15. CAPSID Protein II Protein III Protein IIIa Protein IV Protein VI Protein VIII Protein IX - hexon minor polypeptide - stability/particle assembly CORE Protein V - links DNA to penton base - histone-like, packaging

  16. CAPSID Protein II Protein III Protein IIIa Protein IV Protein VI Protein VIII Protein IX - hexon minor polypeptide - stability/particle assembly CORE Protein V - links DNA to penton base - histone-like, packaging Protein VII - histone-like, packaging

  17. CAPSID Protein II Protein III Protein IIIa Protein IV Protein VI Protein VIII Protein IX - hexon minor polypeptide - stability/particle assembly CORE Protein V - links DNA to penton base - histone-like, packaging Protein VII Protein X (µ) - packaging

  18. CAPSID Protein II Protein III Protein IIIa Protein IV Protein VI Protein VIII Protein IX - hexon minor polypeptide - stability/particle assembly CORE Protein V - links DNA to penton base - histone-like, packaging Protein VII Protein X (µ) 55kD Terminal protein - genomic replication

  19. CAPSID Protein II Protein III Protein IIIa Protein IV Protein VI Protein VIII Protein IX - hexon minor polypeptide - stability/particle assembly CORE Protein V - links DNA to penton base - histone-like, packaging Protein VII Protein X 55kD Terminal protein dsDNA

  20. ATTACHMENT & ENTRY CD46 for Human Adv subgroup B

  21. Disassembly of proteins IIIa, IV, III, VIII Intracellular reducing environment activates viral protease and cleavage of protein VI that links the viral core to capsid

  22. EARLY TRANSCRIPTION & TRANSLATION <8 hr

  23. GENOME REPLICATION

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