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DNA Probe. By: Bentley Hammet, Carly Warfield, Wynn Smythe. Definition of DNA Probe. A molecule labeled with a radioactive isotope, dye, or enzyme that is used to locate a particular sequence of DNA base pairs Also called DNA profiling. What is it made of?.
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DNA Probe By: Bentley Hammet, Carly Warfield, Wynn Smythe
Definition of DNA Probe • A molecule labeled with a radioactive isotope, dye, or enzyme that is used to locate a particular sequence of DNA base pairs • Also called DNA profiling
What is it made of? • A DNA probe is made up of a synthetic sequence of DNA bases that is complementary to a small portion of the DNA strand. • Anywhere that the complementary sequence is found on the DNA, the probe will bind to complementary bases.
What does it do? • DNA probes are used to identify the unique sequences in each person’s DNA • Most of the human DNA is it establishes feet, hands and legs…etc. • DNA probing helps to separate the unique DNA of a certain person
How does it work? • Once a blot,the specific DNA that has been seperated, has been placed on a block of gel--the probe of synthetic DNA placed on the blot of real DNA • The probe will attach itself to the “polymorphic” (the DNA specific to that person) and all other DNA is washed away • From this a DNA fingerprint can be found
When is it used? • It is used to identify specific people through their small fragments of specific DNA. • DNA probing is often times used to help solve crimes, the DNA probing helps to advance and identify suspect DNA
Video • DNA Probe