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Genetic Requirements for DCLs in CMV siRNA Production

This figure displays the detection of CMV siRNAs and genomic RNAs in different plant mutants infected with CMV, highlighting the role of DCL proteins in siRNA production. The results provide insights into the genetic factors influencing antiviral RNA silencing.

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Genetic Requirements for DCLs in CMV siRNA Production

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  1. Suppl Figure 1 dcl2-1 dcl3-1 dcl4-2 dcl3-1 dcl4-2 dcl2-1 dcl4-2 dcl2-1 dcl3-1 A dcl3-1 dcl4-2 dcl2-1 Col0 - + - + - + - + - + - + - + - + 24-nt CMV (-) siRNAs 22-nt 21-nt miR173 U6 B dcl2-1 dcl3-1 dcl4-2 dcl3-1 dcl4-2 dcl2-1 dcl4-2 dcl2-1 dcl3-1 dcl3-1 dcl4-2 dcl2-1 Col0 - + - + - + - + - + - + - + - + RNA1&2 RNA3 RNA4 RNA4A RNA5 25S rRNA 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Figure S1. Genetic requirements for DCLs in the production of CMV siRNAs. (A) Detection of CMV siRNAs from the upper systemically infected leaves harvested at 14 dpi of wild-type Col-0, dcl2-1, dcl3-1,dcl4-2, dcl2-1dcl3-1, dcl2-1 dcl4-2, dcl3-1 dcl4-2 and dcl2-1dcl3-1dcl4-2 plants either mock inoculated (-) or infected with CMV (+). The membrane was also probed for miR173 and U6 RNA. Positions of the 21, 22 and 24 nt siRNAs are indicated. (B) Detection of CMV genomic and subgenomic RNAs (indicated on the right) in the inoculated leaves of the same plants as in (A). 25S rRNA served as a loading control.

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