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Eye banking : corneal recovery. diego.ponzin@fbov.it The Veneto Eye Bank Foundation, Venice. Workshop on Quality Systems in Ocular Tissue Banking , TAIEX 50564 . Zagreb , Croatia, 30-31 January 2013. Corneal donation. Generation of the U nique D onation C ode). Corneal recovery.
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Eye banking: cornealrecovery diego.ponzin@fbov.itThe Veneto Eye Bank Foundation, Venice • Workshop on Quality Systems in Ocular Tissue Banking, TAIEX 50564. • Zagreb, Croatia, 30-31 January 2013
Corneal donation • Generation of the Unique Donation Code)
Corneal recovery Donor maintenance: • Closed eyes, humidification(no ice / ointments / gauges) • Short post morteminterval • Corneal reflex stimulation: gauge, sterile physiologic • Low ambient remperature Ice on donoreyes Corneal reflex stimulation
Corneal recovery • Consent, ascertainment of death, identification • Physical and in situ inspection • Disinfection of peri-ocular tissues, sterile field preparation • Disinfection and rinsing of ocular tissues • Excision/enucleation • Reconstruction • Medical-legal requirements
Dressing • Personal Protective Equipment • Surgical scrub • Always work on a clean and tidy work surface
Physical inspection of the donor (checking for evidence of parenteral drug use, and other high-risk behaviors for AIDS and hepatitis) • Put on personal protective clothing • A visual head-to-toe inspection of each eye donor should be performed, including the webs of fingers and toes, as well as the groin and behind the knees • Look for: • bruises • lacerations, scars, piercing • needle tracks not compatible with recent pathological anamnesis • fresh tattoos that may hide parenteral drug use, and other high-risk behaviors • and signs of transmissable diseases such as Kaposi sarcoma, swollen lymph nodes, skin rashes
Procurement: recovery cases • 100% disposablematerial • 100% traceability
Peritomy, sclerotomy Sclerotomy and recovery: instrumentsdifferent from those for conjunctiva
Sclerotomy NO! • Scleral rim ~ 4 mm • Regular cutting NO!
Corneal-scleralrimdetachment(mostfrequentlyassociated to technicalerrors)
NO! • Noinstruments in the AC • No tension, distorsion
Tell me, and I’llforget; show me, and I mayremember; involve me, and I’llunderstand (OldChineseproverb) Come and visitus! Ponzin D et al. Eye banking at the Fondazione Banca degli Occhi del Veneto. Organs and Tissues2003;111-9 Ferrari S et al. Advances in cornealsurgery and celltherapy: challenges and perspectives for the eyebanks. Expert Review of Ophthalmology2009;4(3):317-29