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François Jacob (right) and Jaques Monod

François Jacob (right) and Jaques Monod. LacI : lactose repressor. LacZ :  -galactosidase LacY :  -galactoside permease LacA : thiogalactoside transacetylase. LacZ’ = alpha fragment located on cloning plasmid LacZ ’’ = omega fragment located in chromosome. X-Gal 5-bromo-4-chloro-3-

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François Jacob (right) and Jaques Monod

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  1. François Jacob (right) and Jaques Monod LacI: lactose repressor LacZ: -galactosidase LacY: -galactoside permease LacA: thiogalactoside transacetylase

  2. LacZ’ = alpha fragment located on cloning plasmid LacZ’’ = omega fragment located in chromosome

  3. X-Gal 5-bromo-4-chloro-3- indolyl--D-galactoside IPTG Isopropyl-thio-galactoside

  4. X-Gal Blue precipitate

  5. http://fajerpc.magnet.fsu.edu/Education/2010/Lectures/25_DNA_Replication_files/image008.jpghttp://fajerpc.magnet.fsu.edu/Education/2010/Lectures/25_DNA_Replication_files/image008.jpg

  6. http://fajerpc.magnet.fsu.edu/Education/2010/Lectures/25_DNA_Transcription_files/image008.jpghttp://fajerpc.magnet.fsu.edu/Education/2010/Lectures/25_DNA_Transcription_files/image008.jpg

  7. http://www.nsci.plu.edu/~mivey/4072002/powerpoint/DNA%20Sequencing/img014.GIFhttp://www.nsci.plu.edu/~mivey/4072002/powerpoint/DNA%20Sequencing/img014.GIF

  8. Navigating the human genome web sites

  9. Starting points: • http://www.ncbi.nlm.nih.gov/entrez/ • http://www.ncbi.nlm.nih.gov/projects/genome/guide/human/ • http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene • http://ca.expasy.org/tools/protparam.html

  10. Reverse transcriptase and repeated sequences

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