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François Jacob (right) and Jaques Monod. LacI : lactose repressor. LacZ : -galactosidase LacY : -galactoside permease LacA : thiogalactoside transacetylase. LacZ’ = alpha fragment located on cloning plasmid LacZ ’’ = omega fragment located in chromosome. X-Gal 5-bromo-4-chloro-3-
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François Jacob (right) and Jaques Monod LacI: lactose repressor LacZ: -galactosidase LacY: -galactoside permease LacA: thiogalactoside transacetylase
LacZ’ = alpha fragment located on cloning plasmid LacZ’’ = omega fragment located in chromosome
X-Gal 5-bromo-4-chloro-3- indolyl--D-galactoside IPTG Isopropyl-thio-galactoside
X-Gal Blue precipitate
http://fajerpc.magnet.fsu.edu/Education/2010/Lectures/25_DNA_Replication_files/image008.jpghttp://fajerpc.magnet.fsu.edu/Education/2010/Lectures/25_DNA_Replication_files/image008.jpg
http://fajerpc.magnet.fsu.edu/Education/2010/Lectures/25_DNA_Transcription_files/image008.jpghttp://fajerpc.magnet.fsu.edu/Education/2010/Lectures/25_DNA_Transcription_files/image008.jpg
http://www.nsci.plu.edu/~mivey/4072002/powerpoint/DNA%20Sequencing/img014.GIFhttp://www.nsci.plu.edu/~mivey/4072002/powerpoint/DNA%20Sequencing/img014.GIF
Starting points: • http://www.ncbi.nlm.nih.gov/entrez/ • http://www.ncbi.nlm.nih.gov/projects/genome/guide/human/ • http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene • http://ca.expasy.org/tools/protparam.html