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Molecular Axon Guidance

Molecular Axon Guidance. PC12 cell…. then NGF added. NGF is neurotropic and neurotrophic. Guidance Factors. Growth Factors. Netrin..was one of the first neurotropic factors. Findings. Netrin from FP. Netrin from FP, But perpendicular. Netrin in media. Cell locomotion like GCs.

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Molecular Axon Guidance

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  1. Molecular Axon Guidance PC12 cell…. then NGF added NGF is neurotropic and neurotrophic

  2. Guidance Factors Growth Factors

  3. Netrin..was one of the first neurotropic factors

  4. Findings

  5. Netrin from FP

  6. Netrin from FP, But perpendicular

  7. Netrin in media

  8. Cell locomotion like GCs Cell moves forward by extending filipodia & lamellipodia Focal adhesions are formed Cell is pulled forward

  9. Diffusible Contact dependent

  10. Extracellular matrix

  11. Extracellular matrix Proteins of ECM - collagen

  12. Extracellular matrix Proteins of ECM - proteoglycans

  13. Extracellular matrix Proteins of ECM - fibronectin

  14. Extracellular matrix collagen Proteins of ECM - laminin laminin

  15. Interaction of cells with ECM inactive INTEGRINS

  16. Interaction of cells with ECM INTEGRINS ligands have RGD motif differentiation motility growth survival functions of outside-in signals

  17. Interaction of cells with ECM FOCAL ADHESIONS FA

  18. Interaction of cells with ECM FOCAL ADHESIONS

  19. Interaction of cells with cells CELL ADHESION MOLECULES

  20. Interaction of cells with ECM and other cells CELL ADHESION MOLECULES VS ECM ATTACHMENT MOLECULES

  21. Ca 2+ Wnt E-Cadherin P Frz II b-catenin p120 a-catenin APC Dsh Axin actin filaments Gsk3b P Tcf/Lef Extracellular Space

  22. Passive Inhibition Rho-GTPases Active Promoter AJ’s Type of Cadherin Stabilize others junctions CDKI: p27/p21 Wnt Motility Proliferation Adhesion Receptor Signaling Actin Cytoskeleton Transcriptional/ Nuclear Role -catenin Rho-GTPases Arp2/3 EGF FGF PDGF -catenin/Wnt P120/kaiso In nucleus Cadherins as Master Regulators of Morphology • Focus is often on adhesive function: Cadherins as molecular glue Cadherins

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