Genetically Engineered Materials Science & Engineering Center

1. Genetically Engineered Materials Science & Engineering Center Mehmet Sarikaya, University of Washington, DMR 0520567 GEPI-Assisted Co-Assembly of Functional Nanostructures • In the fabrication of microarrays, nano-entities are spatially immobilized on an inorganic surface, directly, via various lithography techniques, e.g., soft lithography, dip-pen lithography, and photolithography, all using either silane or thiols as linkers, that have limitations and potential problems; • Using a GEPI-based linker approach (genetically-engineered peptide for inorganic) we developed a versatile procedure for targeted immobilization of nano-entities; • Based on targeted self-assembly, the new approach is applicable for a diversity of biomolecules and nanoparticles, functionalized with GEPI, for a wide range of proteomics, enzymatics, and sensor applications. Fluorescein Biotin QBP1-bio QBP1 QBP1 QBP1-F (i) Co-assembled Architecture (vi) PDMS Stamp 50 µm (ii) Surface contact (iii) SA-QD (v) Directed-self assembly of QBP1-F Directed Self-assembly of SA-QD (iv) T. Kacar et al., Adv. Mater., 21, 295 (2009). Supported by NSF via GEMSEC, MRSEC at UW (DMR-0520567). For more information, visit the Genetically Engineered Materials Science and Engineering Center at: http://depts.washington.edu/gemsec/