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Hypothesis:

Hypothesis:. “Amygdala processing emotional information then used by cortex to drive appropriate behavioral responses to the stimuli”. Background:. Gustatory cortex (GC) and basolateral amygdala (BLA ) are monosynaptically and polysynaptically connected.

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Hypothesis:

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  1. Hypothesis: “Amygdala processing emotional information then used by cortex to drive appropriate behavioral responses to the stimuli”

  2. Background: • Gustatory cortex (GC) and basolateralamygdala (BLA) are monosynapticallyand polysynaptically connected. • GC taste responses progress through sequences of epochs (taste-specific spike rates with particular response latencies and durations), which code first the presence, then the identity, and finally the palatability of tastes. • Response dynamics in BLA are a close match for those of GC, in that BLA neurons switch through epochs at similar times. Palatability-related information appearsoneepoch earlier in BLA

  3. Materials and Methods: • Female Long-Evans Rats served as subjects. Theanesthetizedrat was placed in a stereotaxic frame, its scalp excised, and holes bored in its skull for the insertion of self-tapping ground screws, electrode bundles, and guide cannulae for infusions. • Muscimol was used to temporallly inactivate BLA. Muscimol(100 ng/0.5 l; MP Biomedicals) or saline vehicle was infused bilaterally into BLA at a rate of 0.25l/min for 2 min (total infusate, 0.5l). • Neural signals were collected from GC during taste sampling.

  4. Sample histology showing placement of electrode tips.Sample histology showing placement of cannula tips centered in BLA. basolateral amygdala Insular cortex

  5. The overall impact of BLAx on the entire GC population.

  6. BLAx changes firing rate of responses and recover within 8 h after muscimolinfusion.

  7. Epoch dependency of the effects of BLAx.

  8. Late-epoch palatability processing is specifically affected by BLAx.

  9. Conclusion: Temporary inactivation of BLA (BLAx) halfway through GC recording sessions changed taste responses in all but a handful of GC neurons. BLAxaffected firing rates across entire responses, but the impact was epoch-specific: Late-epoch palatability-related information was abolished or diminished in all but one small, identifiable subset of neurons, while identity-related information was left intact. Thus, BLA plays a powerful role in the driving of palatabilityrelatedresponses in GC, but the relationship between the two structures appears to be complex.

  10. Thanks for attention!

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