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A. Why are enzymes referred to as biocatalysts?

A. An experiment examining an enzyme’s ability to breakdown starch at different temperatures was conducted. Looking at the spot plate at your station, at what temperature was the enzyme least effective?

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A. Why are enzymes referred to as biocatalysts?

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  1. A. An experiment examining an enzyme’s ability to breakdown starch at different temperatures was conducted. Looking at the spot plate at your station, at what temperature was the enzyme least effective? B. Would changing the amount of available enzyme always result in product increase? Why or why not?

  2. A. Why are enzymes referred to as biocatalysts? B. Using the experimental setup below, at what temperature does complete hydrolysis of starch to maltose occur?

  3. The graph below is based on data from an experiment examining the optimal temperature for amylase activity. Use this graph to answer the following questions: A. At what temperature is amylase least effective? B. What is the optimal temperature for amylase activity?

  4. A. This tube’s solution has been tested for sugars. Does it contain very low, moderate, very high concentration of sugar or no sugar? B. True or false. The organic molecule in this tube is a monosaccharide.

  5. A. Given the set up at your station, which tube do you think contains lipids and why? B. What test can be used to detect the presence of lipids?

  6. A. You and your lab partner are assigned to identify 1 unknown macromolecule sample by using the Biuret, Benedicts, Iodine, and Sudan IV tests. Unfortunately, after running the test he/she leaves and has not written down what test belongs to each tube. Based on the results below, what type of macromolecule is this? B. What was she testing for with sample 1?

  7. A. Is this organism at your station a prokaryote or a eukaryote? B. Give one defining characteristic of a prokaryote.

  8. A. Is the cell pictured below a prokaryote or eukaryote? B. What part of the cell’s structure tells you this (answer to part A)?

  9. A. Given the conditions within the dialysis bag labeled D, which direction would you expect the water to move? B. The solution within this bag is considered to be ___________ a) Isotonic b) Hypotonic c) Hypertonic d) None

  10. A. From the data set provided, calculate the mean. B. Standard deviation is defined as the square root of the ____________.

  11. Data Set 1: 6, 9, 3, 4, 4, 1, 1, 1 Data Set 1: 6, 6, 30, 5, 9, 3, 3, 6 Data Set 1: 2, 4, 5, 1, 3, 3, 5, 3 Data Set 1: 9, 9, 7, 6, 7, 7, 7, 2 Data Set 1: 10, 11, 15, 3, 3, 9

  12. A. Given a field of view in your low power objective = 5mm, magnification at low power= 4X, and magnification at high power = 10X, what is your field of view at high power? B. What is the magnification of the objective lens labeled X?

  13. A. How many milliliters are in 14 liters? B. Where should volume of a liquid be read in relation to the meniscus that forms on the surface?

  14. A. How many milliliters are in 14 liters? B. Where should volume of a liquid be read in relation to the meniscus that forms on the surface?

  15. 2-Naphthylamine (2-Nap), a starting material used in dye synthesis, was • employed commercially in 1890. However, soon after the implementation of 2-Nap factory workers began to develop bladder cancer. A federal agency was appointed to determine whether 2-Nap exposure was the causative agent of factory workers’ bladder cancer. In one of the studies performed by the agency to assess this issue, 8-month old female rats were exposed to varying concentrations of 2-Nap. The results generated by this study are presented in the graph below: • Formulate an appropriate null hypothesis for this experiment. • Based on the data displayed in the graph, do you reject or fail to reject your null hypothesis? Explain your answer.

  16. A large-scale survey reported an increase in the incidence of skin • cancer in people who wear sunblock lotions containing zinc-oxide. To determine if zinc-oxide influences the risk of developing skin cancer, an experiment was conducted in which 60 albino male rats were treated daily with varying concentrations of zinc-oxide solution and the incidence of skin cancer was evaluated. The results of this study are illustrated graphically below: • Formulate an appropriate null hypothesis for this experiment. • Based on the data displayed in the graph, do you reject or fail to reject your null hypothesis? Explain your answer.

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