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By Group #3 Phillip Keller Micheal Jones Krista Melish James Kancewick

Nanotech in Biomedicine: Biodegradable Polymer Nanoparticles for drug delivery through mucosal Membranes. By Group #3 Phillip Keller Micheal Jones Krista Melish James Kancewick. Overview. Background and Introduction Nanotech in Biomedicine Problem and Basic Principles

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By Group #3 Phillip Keller Micheal Jones Krista Melish James Kancewick

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  1. Nanotech in Biomedicine: Biodegradable Polymer Nanoparticles for drug delivery through mucosal Membranes By Group #3 • Phillip Keller • Micheal Jones • Krista Melish • James Kancewick

  2. Overview • Background and Introduction • Nanotech in Biomedicine • Problem and Basic Principles • Nano Particle Immobility/Transport Process • Experiment and Results • Polymer Prep/Transport Characteristics • Improvements and Future Research

  3. Introduction • Nanotechnology • Very Small • Very Powerful • Many Applications • Rapid Research Growth http://www.redicecreations.com/ul_img/2353nanotechnology.jpg

  4. Introduction: Biomedicine • Biomedicine • Drug delivery • Biodiagnostics • Biosensors • Bioimaging http://www.tlc2.uh.edu/Research/Institutes_Centers/visual_logo.jpg

  5. Drug Delivery • Selective Targeting • Organs • Pathogens • Cancer Cells • Protects Deliverable • Less Medicine Wasted • Controllable Release http://web.mit.edu/newsoffice/2008/nanoparticles.jpg

  6. Biodiagnostic • Detection of Nucleic Acids • PCR (Polymerase chain reaction) • Detection of Proteins • High levels of certain proteins are indicative of various cancers and diseases http://www.npl.co.uk/upload/img_200/ AB_Still_z_0022_prv.jpg

  7. Biosensors http://life.nctu.edu.tw/~cslin/Tw/Research/Biosensing%20System.files/image003.gif

  8. Bioimaging • Enhance Current Imaging Techniques • Compliment MRI/CT • Provide contrast in previously unobtainable areas • Reduce need for exploratory surgery http://www.feilab.org/pictures/facility/Flurosecence_Results.jpg

  9. Problems with Nano Particle Drug Delivery 100X Salival Mucus http://www.tryingtoconceive.com/store/fern1.jpg

  10. Mucus Penetration Cervicovaginal Mucus Fibers • Cystic Fibrosis • Thickening of lung mucosal layers • Eye • Gut • Drug delivery to impede cancer growth • Female Reproductive Diseases http://www.sciencedirect.com/science?_ob=ArticleURL

  11. Mucus Penetration http://www.sciencedirect.com/science?_ob=MiamiCaptionURL&_method=retrieve&_udi=B6T3R-4V4KPJ9-3&_image=fig6&_ba=6&_user

  12. Particle Biodegradability and Drug Delivery • No Interaction with Mucus Fibers • Dense PEGylation of particle surface allows mucus penetration due to neutral charge distribution http://www.sciencedirect.com/science?_ob=MiamiCaptionURL&_method=retrieve&_udi=B6T3R4V4KPJ93&_image=fig8&_ba=8&_user=952835&_coverDate=02%2F27%2F2009&_rdoc=1&_fmt=full&_orig=search&_cdi=4953&_issn=0169409X&_pii=S0169409X08002652&view=c&_acct=C000049198&_version=1&_urlVersion=0&_userid=952835&md5=be4fd7ee848e89aca12a6089c26869d2

  13. Biodegradable Nanoparticle With Encapsulated Drug

  14. Synthesis of PSA-PEG • One Step Formulation Process • Diblock Copolymers • Covalently Bonded

  15. Mucus-Penetrating Nanoparticle • Dense Surface Coating of PEG • Low Molecular Weight • Neutral Surface Charge • Non-Penetrating particles have highly negative surface charge

  16. Flexible Drug Delivery • Controlled Drug Release • Vary ratio of PEG to PSA to alter particle degradation rate

  17. Physicochemical Properties of Nanoparticles • Zai Potential • Surface charge characteristic • Dw/Dm • Ratio of dispersion coefficients for particle in water compared to human cervical-vaginal mucus

  18. Results & Conclusions • Rapid Transport • Can Penetrate CVM Rapidly

  19. Penetration in Cystic Fibrosis Sputum • Rapid Penetration of CF Sputum

  20. Improvements and Further Research • Increase Particle Transfer Rates • Adapt to more specific tissues http://www.intel.com/technology/itj/2008/v12i3/3-paper/figures/Figure6.gif

  21. Improvements and Further Research • Develop Applications for a Wider Range of Diseases • Create nanoparticles capable of accepting a wider array of drugs • Develop more chemically gentle manufacturing processes for coating particles in PEG http://www.labgrab.com/files/pictures/users/blogs/HIV.jpg

  22. Improvements and Further Research • Reduce Cost of Production • Make economically viable • Increased targeting reduces dosage and drug waste http://www.maserati-rc.org/PicLib/Factory_new_production_line_3.jpg

  23. Questions?

  24. Resources • Tang, Benjamin, Michelle Dawson, Samuel Lai, Ying-Ying Wang, Jung Suk, and Ming Yang. "Biodegradable polymer nanoparticles that rapidly penetrate the human mucus barrier." Proceedings of the National Academy of Sciences 106.46 (2009): 19268-9273. • Various websites listed in slides

  25. Rebuttal From Group U3 Micheal Jones Phillip Keller Krista Melish James Kancewick media.podcasts.ox.ac.uk/mat/nanotechnology/nanotechnology_albumcover.jpg

  26. What we did well • Confidence • Graphics • Well Dressed • Basic Intro • More in depth article http://www.ipcom.co.nz/tl_files/images/Diver-Businessman-web.jpg

  27. Areas For Improvement • Elimination of filler words • Increasing presentation time and content • Better eye contact with the audience • Relabeling graphics with small words • Increase group understanding of the article http://www.ipcom.co.nz/tl_files/images/Diver-Businessman-web.jpg

  28. Biodegradable Polymer Nanoparticles for drug delivery through mucosal MembranesGroup U1 • The overall presentation was very good and interactive with the audience. • The nanoparticle PSA-PEG showed to be a very effective polymer since it has good diffusion through some specific organic membranes • Further research can be done on improving the efficiency in delivering drugs • Its still a problem to power this particles. • We still have to do work on targeting the right receptors because in body we may have several similar mucosal membranes with similar biological and chemical properties that are healthy. http://science-hub.com/2009/06/platinum-particles-in-the-form-of-japanese-sweets/

  29. Review of Group U3’s Presentation- Biodegradable Polymer Nanoparticles for Drug Delivery through Mucosal Membranes By Group U2: -Kyle Demel -Keaton Hamm -Bryan Holekamp -Rachael Houk http://dipc.ehu.es/nano2006/Nanoparticles.jpg http://radio-weblogs.com/0105910/ images/nanoparticles.jpg

  30. The presenters could have improved in the following areas: • Presentation too short: • 30-minute presentation only lasted 15 • Needs elaboration on further research: • Delivery with viral protein coatings • Transfer through cellular membranes • Explain the pictures on the slides; do not just add pretty pictures because you have to • Oral delivery • Practice speech • Several group members were not knowledgeable on article • Excessive use of filler words • Work on timing and transitions http://www.ediblecomputerchips.com/Nano-Tech/Final/Seven-A.jpg http://news.softpedia.com/images/news2/How-Dangerous-Are-Nanoparticles-2.jpg

  31. Nanotech in Biomedicine: Biodegradable Polymer Nanoparticles for drug delivery through mucosal Membranes Reviewed by Group U4

  32. Presentation/Oral Review • Overall, the speakers sounded very confident, thus enabling the audience to stay attentive to the information presented as opposed to the flaws in speech. • Excellent slides with a good balance of text and graphics. Both were large enough for the audience in the back to see clearly • Nice hands-on example with the grapefruit/lemons for further explanation of the topic.

  33. Technical Review • Perhaps this is because this research is so new, but we would have liked to have a more in depth description on how the mucus penetration would work in a variety of applications. • Also, maybe devote a little time to the future of this technology(different diseases it could fight, and different barriers the nanoparticle could help break through other than the mucus) • Overall however, good presentation that left us with few question. The questions we did have were answered well.

  34. Nanotech in Biomedicine :Biodegradable Polymer Nanoparticlesfor drug delivery through mucosal Membranes • Review of Team U3 by Team U5 – Jaynesh Shah, Greg Pudewell, Edwin L. Youmsi Pete and John Pack.

  35. Oral and Quality of Slides Review • Speakers did a good job in the oral aspect of the presentation. They were loud and clear confident. Implying they had done a good amount of research on the topic. • The slides clear, visible and not overcrowded. Team U5 made good use of several pictures, images and graphs.

  36. Technical Review • The presentation was satisfying from a technical standpoint. • We would have however liked them to discuss the use of biosensors in a little more details with respect to the issue of drug delivery

  37. Nanotech in Biomedicine: Biodegradable Polymer Nanoparticlesfor drug delivery through mucosal Membranes Presented By Group #3 Phillip Keller Micheal Jones Krista Melish James Kancewick Critiqued by: Group U6 - Pavitra Timbalia, Michael Trevathan, Jared Walker

  38. Review – Slides and Oral Presentation • The slides were well balanced with graphics and text. • All presenters were well dressed and maintained an appropriate tone. • The demonstration involving the fruit to describe the receptors of drugs helped to open the presentation and get the audiences attention. • A few of the graphs were difficult to read and understand, and were not explained carefully. • The evolution leading to the development of PEG wasn’t explained until someone asked about it during the questions section.

  39. Review - Technical • This presentation dealt with the delivery of drugs through the use of nano-particles engineered specifically to be able to penetrate through thick mucus that was previously not possible. • Future research needs to be done to test the particles ability to deliver drugs • The tests done for the paper only showed that the particles were able to penetrate the mucus - they didn’t contain any drug payload. • This technology could help to treat many diseases such as cystic fibrosis, provided future research is done to reduce the cost of production.

  40. Review for U3from G6 Jung Hwan Woo

  41. The table on the top left shows the diffusivity of the nanoparticle in mucus compared to the diffusivity in water. It is used to indicate the rate at which the nanoparticle travels in the body through mucus. • What are the factors that affects these rates? • What can be done to improve the particle transfer rate as suggested on the bottom left? Jung Hwan Woo

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