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The plate cells are coated with rabbit antibodies to estradiol.

The plate cells are coated with rabbit antibodies to estradiol. ANTIBODIES. Stds and Unks compete with estradiol linked to horseradish peroxidase in enzyme conjugate for antibody binding sites. E-HP. x. x. x. x. x. x. x. x. x. x. x. x. x. x. x. x. x. x. x. x. x. x. x. x.

mariko-phillips
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The plate cells are coated with rabbit antibodies to estradiol.

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  1. The plate cells are coated with rabbit antibodies to estradiol. ANTIBODIES Stds and Unks compete with estradiol linked to horseradish peroxidase in enzyme conjugate for antibody binding sites. E-HP x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x E x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x E-HP x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x E-HP x x x x x x x x x x E E E-HP The bound estradiol peroxidase is measured by its reaction to Tetramethylbenzidine (TMB), producing a blue color. TMB E-HP E E E E E-HP E-HP E-HP E-HP E E E E E-HP E E-HP E-HP After stopping the Rx with sulfuric acid, color turns yellow. H2SO4 E E x x x x x Spectroradiometric plate reader measures optical density of cells at 450 nm and at 630 nm for correction. @ of estradiol peroxidase detected, which is inversely proportional to the @ of estradiol present.

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