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ERCC5. 30. DDB1. 35. 25. 30. Control. Control. 25. 20. UVB. UVB. 20. % Total RNA. 15. % Total RNA. 15. 10. 10. 5. 5. 0. 0. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Fraction. Fraction. ERCC1. 50. 40. Control. UVB. 30.
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ERCC5 30 DDB1 35 25 30 Control Control 25 20 UVB UVB 20 % Total RNA 15 % Total RNA 15 10 10 5 5 0 0 2 3 4 5 6 7 8 9 10 11 2 3 4 5 6 7 8 9 10 11 Fraction Fraction ERCC1 50 40 Control UVB 30 % Total RNA 20 10 0 2 3 4 5 6 7 8 9 10 11 Fraction c-Myc 25 Control 20 40 XPD UVB 15 Control % Total RNA 30 UVB 10 % Total RNA 20 5 10 0 2 3 4 5 6 7 8 9 10 11 Fraction 0 Ape1 2 3 4 5 6 7 8 9 10 11 20 Fraction Control 15 UVB % Total RNA 10 rps16 35 5 30 25 0 % Total RNA 2 3 4 5 6 7 8 9 10 11 20 Fraction 15 10 5 0 2 3 4 5 6 7 8 9 10 11 Fraction OGG1 35 30 Control 25 UVB 20 % Total RNA 15 10 5 0 11 2 3 4 5 6 7 8 9 10 Fraction Quantification of Northern analysis shown in Figure 2A The Northern blots obtained in figure 2A were analysed using Image Quant and the relative amount of RNA in each fraction was calculated and expressed relative to the total amount of RNA. Fractions 4,5, and 6 correspond to the positions of the 40S, 60S and 80S ribosomal subunits and fractions 6-11 correspond to the polysomes. Supplementary 3