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EIAR. Developing Gender Responsive Community Based Low Cost Tissue Culture Innovations for Improved Food Security and Livelihoods in the ECA. Belayneh A., Ibsa F. & Abel D. EIAR: Agricultural Biotechnology Research Programme Four Centers: Holetta , Melkassa , Jimma & DZ TC Labs
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EIAR Developing Gender Responsive Community Based Low Cost Tissue Culture Innovations for Improved Food Security and Livelihoods in the ECA Belayneh A., Ibsa F. & Abel D.
EIAR: Agricultural Biotechnology Research Programme Four Centers:Holetta, Melkassa, Jimma & DZ TC Labs Major Crops:Enset, Potato, SP, Apple, Pineapple, Coffee, Banana, SC, Grape, garlic, etc
POTATO Currently supplying plantlets to the Aeroponic unit
Enset Conventional = 2-3 years old sucker Produce 30-50 plantlets per sucker Tissue Culture = 1 – 2 months old suckers Produce 40 plantlets per sucker
Haploid production by TC Tef, Noug & Brassica
Sweet Potato Importance One of the most important root and tuber crops: enset, potato & SP 95% grown in south, SW and east of the country – co-staple food Recent introduction in the north and NW – widely accepted A potential as food & feed - Food security crop Production = 40,000 ha = 4 million tons
Major production constraints: • Aattackedby spweevil, spbutter fly and beetle • It alsogetsattacked by blights, soft rot and virus diseases • FMV highly distructive • Distribution of potato cuttings halted • Virus cleaning protocol developed
Clean Sweet potato multiplication Acclimatization In vitro propagation Virus Indexing Farmers Virus Indexing Further propagation
Banana Importance Cash crop for small scale farmers Nutritious Produced in the south and SW of the country Production = 23,570 ha = 207,000 tons
Conventional Multiplication Small rate of multiplication A maximum of 12 suckers/year/mother plant High variability among suckers – management problems Disease transmission Bulkiness – difficult to transport
Tissue Culture High and fast multiplication – 1000 plantlets/explant/year Young suckers required: 3 - 5 months old Disease free Uniform and vigorous plantlets A protocol optimized for three varieties at Melkassa: Poyo, DC, GC A protocol under optimization for other varieties: William 1, Grandne, Butuzuwa