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Alejandro González-Serna 1,2 , Ezequiel Ruiz-Mateos 2 , Manuel Leal 2 and Alejandro Vallejo 1,3 .

Abstract no. CDA020. Analysis of the alterations of T-cell receptor repertoire in HIV-infected patients who underwent treatment interruption. Alejandro González-Serna 1,2 , Ezequiel Ruiz-Mateos 2 , Manuel Leal 2 and Alejandro Vallejo 1,3 .

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Alejandro González-Serna 1,2 , Ezequiel Ruiz-Mateos 2 , Manuel Leal 2 and Alejandro Vallejo 1,3 .

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  1. Abstract no. CDA020 Analysis of the alterations of T-cell receptor repertoire in HIV-infected patients who underwent treatment interruption Alejandro González-Serna1,2, Ezequiel Ruiz-Mateos2, Manuel Leal2 and Alejandro Vallejo1,3. 1 Laboratory of Molecular Virology and 2 Laboratory of Immunovirology, Infectious Diseases Service, Virgen del Rocio University Hospital, Seville, Spain. 3 Laboratory of Molecular Virology, Infectious Diseases Service, Ramón y Cajal University Hospital, Madrid, Spain

  2. There is limited information of the alterations of the T-cell receptor repertoire in HIV-infected patients who have had a period of treatment interruption (TI). Our objective was to evaluate the evolution of the repertoire during treatment interruption and the capacity of HAART to recover the repertoire in such patients. Background

  3. Methods • We studied the T cell receptor (TCR)  chain transcript mobilization of CD8+ T cells isolated from ten HIV-1-infected individuals who underwent a period of guided TI. We analysed the patients in three different moments; before TI, at least one year after TI, and at least one year after treatment resume. • This study was performed by using a combined qualitative and quantitative assessment of V mRNA alterations at the level of complementary determining region 3 length distribution (CDR3-LD) of the TCR. The Wilcoxon signed rank test was applied to examine statistically significant differences of CDR3 complexity scores between subpopulations of different CD8+ expression and statistical analysis was performed with the SPSS software version 16.0.

  4. Results Figure 2: TCRV 28: Figure 1 • Patients displayed significant alterations of their V CDR3-LD profile consistent in an increased transcript accumulation of families 10, 14 and 15 after TI and a later decreased of the same families after HAART resume. Other families displayed a significant decreased transcript accumulation of families 20, 28 and 29, after TI and a later increased transcript accumulation of the same families after HAART resume (Figure 1). • These alterations seem to be more pronounced in patients who were longer time under HAART before TI (Figure 2).

  5. Conclusion • Our data suggest a possible common pattern of the V repertoire after TI and this pattern could be more pronounced in patients who were longer time under HAART, maybe because these patients have been more time in contact with the virus. These specific alterations reverted after HAART resume.

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