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TOXICOLOGICAL SCIENCES 78, 258–266 (2004) DOI: 10.1093/toxsci/kfh065

2,3,7,8-Tetrachlorodibenzo- p -Dioxin Inhibits Regression of the Common Cardinal Vein in Developing Zebrafish. 學號： M93360041 學生：朱國榮. TOXICOLOGICAL SCIENCES 78, 258–266 (2004) DOI: 10.1093/toxsci/kfh065 Advance Access publication February 19, 2004. Ⅰ.CCV 為成對生長的血管，環繞於卵黃的四周，並和心臟連接，藉

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TOXICOLOGICAL SCIENCES 78, 258–266 (2004) DOI: 10.1093/toxsci/kfh065

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  1. 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Inhibits Regression of theCommon Cardinal Vein in Developing Zebrafish 學號：M93360041 學生：朱國榮 TOXICOLOGICAL SCIENCES 78, 258–266 (2004) DOI: 10.1093/toxsci/kfh065 Advance Access publication February 19, 2004

  2. Ⅰ.CCV為成對生長的血管，環繞於卵黃的四周，並和心臟連接，藉 由TCDD活化AHR signaling，造成common cardinal vein (CCV) 的發育發生了改變。 Ⅱ.TCDD在卵受精44小時的時候，很明顯的抑制了胚胎CCV的生長， 而在受精62小時後，相對與正常生長的胚胎，CCV的生長約減少 了百分之六十三。 Ⅲ.而在卵受精80小時之後，其反而抑制了CCV的regression，而造 成“string-like”的心臟。因而可了解TCDD的毒性對育胚胎發 育過程中，心血管生成所造成的影響。

  3. Regression of the CCV from 74 to 98 hpf.

  4. The zfahr2 morpholino had no effect on CCV regression in the absence of TCDD, but did protect against the TCDD-induced block of CCV regression. This demonstrates that the TCDD-induced block in CCV regression is AHR2 dependent.

  5. zfahr2-MO treatment protected against the TCDD-induced block of CCV regression. CCV

  6. TCDD為一種環境賀爾蒙，TCDD和AHR結合之後，和其它transcription factor結合，開啟其下游基因的表現，但根據研究指出，TCDD並非只開啟單一的基因調控機制，故尋找和AHR結構相似的蛋白質，進而瞭解此一蛋白質和TCDD的親和性以及其與TCDD產生交互作用後，開啟或抑制其下游基因的表現，進而了解此一蛋白質在胚胎發育的過程中所扮演的角色。 （1GYE）

  7. THX!!

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