1 / 24

Biological Acquisition Unit

Biological Acquisition Unit. Team Members : Fred Avery Ny ‘ Jaa Bobo Gene Council Salvatore Giorgi Advisors: Dr. Helferty Dr. Pillapakkam. Outline of Presentation . Mission Overview O bjective Theory Background / Previous R esearch Biological Analysis Success Criteria Design

marsha
Download Presentation

Biological Acquisition Unit

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Biological Acquisition Unit Team Members: Fred Avery Ny ‘JaaBobo Gene Council Salvatore Giorgi Advisors: Dr. Helferty Dr. Pillapakkam

  2. Outline of Presentation • Mission Overview • Objective • Theory • Background / Previous Research • Biological Analysis • Success Criteria • Design • Constraints • Design Process • System Architecture • Electrical System • Filtration System • Block Diagram • Design Compliance • Management • Team Members • Advisors • Budget Outline

  3. Mission Overview

  4. Objective • Measure the earth’s magnetic field as a function of altitude. • Take biological samples in stratosphere and lower mesosphere. • Measure flight dynamics of the rocket.

  5. Theory • An inertial measurement unit (IMU) is an electrical device consisting of accelerometers and gyroscopes that are used to measure the rocket’s flight dynamics (roll, pitch, and yaw). • The magnetometer will measure the strength and direction of the earth’s magnetic field. • The filtration system will collect organic and inorganic material suspended in the atmosphere.

  6. Background • In 2006, the European Science Foundation funded an exploratory workshop on “Microbiological Meteorology” at the French National Agronomic Research Institute (INRA) in Avignon. • Potential roles of micro-organisms • Act as cloud condensation nuclei and to participate in radiative forcing. • Many airborne micro-organisms likely metabolize chemical components of aerosols thereby modifying atmospheric chemistry.

  7. Previous Research • In 2008, a study identified bacterial species Bacillus subtilis, Bacillus endophyticus, and the fungal genus Penicillium. • In 2005, a study showed about 25% of the particles suspended in air in the size range of 0.2 to 50 μm are primary biological aerosol particles.

  8. Biological Analysis • DAPI • DAPI (6-diamidino-2-phenylindole) is a stain used in fluorescence microscopy. DAPI passes through cell membranes therefore it can be used to stain both live and fixed cells. • BRDU • Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is used for detecting actively dividing cells. • Genetic Sequencing • Determines the number of nucleotides in sample’s DNA: adenine, guanine, cytosine, and thymine • Scanning Electron Microscope • Scans the sample and re-generates image to be analyzed, i.e. structural analysis of microbes

  9. Success Criteria • Acquire Stratospheric specimen • Collect a statistically significant sample to compare to previous studies. • Amount of samples • Type of microbes • IMU (Inertial Measurement Unit) • Accurately and reliably record data such as: • Velocity • Flight Dynamics • Gravitational Force • Magnetometer • Study magnetic field in upper atmosphere. • Compare experimental magnetic field to actual values .

  10. Design

  11. Constraints • The weight of the canister must not be more than 20+0.2lbs. • No power until after takeoff. • The center of gravity should lie within the 1x1x1 inch (x, y, z) envelope of the centriod of the payload canister. • Maximum height is 4.75 inches and maximum diameter is 9.3 inches. • No Lithium rechargeable batteries.

  12. Design Process Schematics, Module Placement on Plate chipKIT Max 32, magnetometer, G Switch, SD card, SD reader, NPT valve, filter canister, filter paper, IMU Prepare Functional Design Confirm System Functionality Data Acquisition Purchase Materials Test Separate Components Compatibility, functionality, defects

  13. System Architecture 2 9 VOLT BATTERIES MAGNETOMETER PIC32 MICROCONTROLLER G- SWITCH IMU Valve for Bio-Filtration System 2 GB SD CARD and READER KEY Power Data

  14. Pic32 ChipKIT Microprocessor Flash Memory: 512K RAM Memory: 128K Operating Voltage: 3.3V Operating Frequency: 80 MHz Typical Operating Current: 90 mA Input Voltage: 7 to 15V Input Voltage (maximum): 20V I/O Pins: 83 Analog Inputs: 16 Analog Input Voltage Range: 0V to 3.3V DC Current Per Pin: +/- 18 mA

  15. Triple Axis Magnetometer HMC584 Power: 2.5 to 3.3 V Field Range: +/- 4 Gauss Current: 0.9 mA Bandwidth: 10 kH Weight: 50 mg

  16. Atomic IMU - 6 Degrees of Freedom Triple-axis accelerometer Sensitivity: 1.5 g, 2 g, 4 g or 6 g Single axis 300°/s gyroscope Voltage: 3.4 to 10 V Current: 24 mA Bandwidth: 150 Hz (Z axis) 350 Hz (X and Y axis)

  17. Filter System • Connects to two ports: Static and Dynamic • Dynamic port draws in samples • Air flow exits through the static port • Contains four filters in series • Filters are decreasing in size from 5 to 0.2 μm • Filter system terminates with NPT connector at each end.

  18. Physical System

  19. Design Compliance • Predicted final mass is 10+0.2 lbs • IMU, magnetometer, batteries, SD card / reader, and microprocessor weigh less than 2 lbs • Projected filtration system weight is less than 2 lbs • More weight needed • Payload Activation • G-switch • Open circuit until g-switch activation

  20. Management

  21. Team Members Fred Avery (ME) • Filtration System • Center of gravity testing • Mass Flow Rates • Ny ‘JaaBobo (EE) • Hardware • Magnetometer • IMU • Power • Gene Council (EE) • Hardware • Magnetometer • IMU • Chip programming • Salvatore Giorgi (ECE) • Team Leader • Microprocessor • Data Acquisition • Filtration System

  22. Advisors Electrical Dr. John Helferty Department of Electrical and Computer Engineering Mechanical Dr. ShriramPillapakkam Department of Mechanical Engineering Biological Dr. Erik Cordes Department of Biology

  23. Parts List / Budget

  24. Conclusion • Issues • Integrating filtration system with canister • Correctly implementing the SD card with the microprocessor • Concerns • Transporting samples from Virginia to Pennsylvania • Properly sterilizing the filtration system • Shared canister logistics • Future Plans • Order microprocessor, IMU, and magnetometer • Design and build filtration system

More Related