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Yifeng Zhou Ph.D

New Wine in Old Bottles A New Approach of SNP Function Research. Soochow University. Yifeng Zhou Ph.D. zhouyifeng@suda.edu.cn. Why We Design This Study?. An Unfinished Story: SNP in TIMP2 Promoters and Risk of Breast Cancer. 1. What Did We Publish Before ?. 2.

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Yifeng Zhou Ph.D

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  1. New Wine in Old BottlesA New Approach of SNP Function Research Soochow University Yifeng Zhou Ph.D zhouyifeng@suda.edu.cn

  2. Why We Design This Study? An Unfinished Story: SNP in TIMP2 Promoters and Risk of Breast Cancer 1 What Did We Publish Before? 2 What Did We not Publish Before? What Did We Perform Now? 3 What Did We Publish Now? 4

  3. My First Paper Zhou et al.Carcinogenesis. 2004 25:399-404.

  4. Backgroud TIMP-2: An Endogenous Inhibitor of MMP-2 TIMP-2 binds with Pro-MMP-2

  5. Result I Zhou et al.Carcinogenesis. 2004 25:399-404.

  6. Result II Zhou et al.Carcinogenesis. 2004 25:399-404.

  7. SNPs in Promoter Real-Time PCR… EMSA Report Gene ChIP Function Assays of SNPs in Promoter

  8. EMSA Data Unpublished

  9. Mechanism Aishan HAN et al. ANALYTICAL SCIENCES 2006

  10. Mechanism Huan Chen et al. Biosensors and Bioelectronics 24 2009 1955–1961

  11. Material Graphite Glassy Carbon

  12. IM6 system

  13. Mechanism

  14. Results • Cyclic voltammograms (scan rate:100mVs-1) • (b) Electrochemical impedance spectroscopy (Nyquist plots) response at different modified process in the electrode

  15. Mechanism

  16. Results

  17. Results Differential pulse voltammograms of [Fe(CN)6]3-/4-accumulated on the probe after its interaction with different concentration of the wtDNA

  18. Results

  19. Results The washing ratio responses as a function of NaCl concentrations in washing buffer

  20. Results

  21. Results EMSA Report Gene G Allele significant higher than C Allele No Difference Between two Allele TIMP-2 -418G/C Breast Cancer Electrochemistry Case-Control Affinity of G is significant higher than C P = 0.060

  22. Conclusion • Present study provides further evidence that the functional SNP in TIMP-2 promoter is genetic susceptibility factors for breast cancer.

  23. Publications Gao Y et al. Carcinogenesis. 2009 30:2064-9.

  24. Yu Xiao Jiang Lan Zheng Jian Kang Shenhui Peng Rui Xu Jinhai Acknowledgment

  25. Thank You! zhouyifeng@suda.edu.cn

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