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Biofuel Cells Purifying engineered enzymes to generate electricity

Biofuel Cells Purifying engineered enzymes to generate electricity . Tila Hidalgo Alief ISD Hastings High School Zhilei Chen, PhD Artie McFerrin Department Department of Chemical Engineering. Biofuel Cells. The Research Objective . Bacterial Transformation and Protein Purification.

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Biofuel Cells Purifying engineered enzymes to generate electricity

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  1. Biofuel CellsPurifying engineered enzymes to generate electricity Tila Hidalgo Alief ISD Hastings High School Zhilei Chen, PhD Artie McFerrin Department Department of Chemical Engineering

  2. Biofuel Cells

  3. The Research Objective

  4. Bacterial Transformation and Protein Purification. • A project for my AP Biology students. http://media.studienet.dk/images/products/medium/106750.jpg My Classroom Project:

  5. In addition to lab skills based around the inquiry model of investigation that is part of the AP Biology curriculum the following college board objectives will be covered: • Essential knowledge 4.A.1: The subcomponents of biological molecules and their sequence determine the properties of that molecule. • Essential knowledge 2.B.2: Growth and dynamic homeostasis are maintained by the constant movement of molecules across membranes. • Essential knowledge 3.A.1: DNA, and in some cases RNA, is the primary source of heritable information. • Essential knowledge 3.C.1: Changes in genotype can result in changes in phenotype. • Phenotypes are determined through protein activities. To foster student understanding of this concept, instructors can choose an illustrative example such as: • Enzymatic reactions • Transport by proteins • Synthesis • Degradation • Genetic engineering techniques can manipulate the heritable information of DNA and, in special cases, RNA. To foster student understanding of this concept, instructors can choose an illustrative example such as: • Electrophoresis • Plasmid-based transformation • Restriction enzyme analysis of DNA • Polymerase Chain Reaction (PCR) College Board Objectives Addressed

  6. Protein Purification Objective:

  7. Day 1

  8. Students will perform the bacterial transformation. I plan to use the BioradpGlo transformation. http://3.bp.blogspot.com/_voJSmT_yBgY/SW6KsV-pvxI/AAAAAAAACA0/ZZWIX5HNA9c/s320/pglomap.gif http://media.studienet.dk/images/products/medium/106750.jpg Day 2-3

  9. Day 4-6

  10. Day 7-8- “Lab Meeting”

  11. Day 9-11- Protein Purification. www.bio-rad.com/webroot/web/software/.../pGLO_GFP_031208.pp

  12. Day 9-11 www.bio-rad.com/webroot/web/software/.../pGLO_GFP_031208.pp

  13. Day 12-13

  14. Rubric Rough Draft

  15. TAMU E3 program • National Science Foundation • Nuclear Power Institute • Dr. Chen and Dr. Guan • Chris Skinner Acknowledgements

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