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A8-023287

A8-023287. Tamas Nagy, DVM, PhD Department of Pathology College of Veterinary Medicine University of Georgia. A8-023287. Signalment and history 3-month-old female IL-10 knock-out mouse Kept in an individually ventilated microisolator cage with one other female and one male

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A8-023287

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  1. A8-023287 Tamas Nagy, DVM, PhD Department of Pathology College of Veterinary Medicine University of Georgia Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  2. A8-023287 • Signalment and history • 3-month-old female IL-10 knock-out mouse • Kept in an individually ventilated microisolator cage with one other female and one male • Gave birth 7 days ago, but all the offspring died • No previous reproductive problems • Developed rectal prolapse 24 hours prior to euthanasia Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  3. A8-023287 • Gross pathology • Rectal prolapse • Gas-filled and moderately dilated proximal colon • Moderately thickened proximal colonic wall Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  4. A8-023287 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only Image courtesy of Dr. R. McManamon

  5. A8-023287 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  6. A8-023287 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  7. A8-023287 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  8. A8-023287 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  9. A8-023287 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  10. A8-023287 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  11. A8-023287 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  12. A8-023287 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  13. A8-023287 • Morphologic diagnosis • Colon: chronic, moderate, granulomatous colitis • Rectum: Rectal prolapse with secondary acute edema and serocellular crust formation Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  14. A8-023287 • Discussion • IL-10 (interleukin 10) • Member of the cytokine family (involved in regulation of T lymphocyte activity) • Produced by T regulatory lymphocytes, TH2 lymphocytes, and dendritic cells • Important mediator in TH2-biased immunologic response – diffuse (lepromatous) granulomas • Johne’s disease • Human leprosy • Crohn’s disease Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  15. A8-023287 • Discussion – continued • IL-10 knock-out (IL-10 KO) mouse • Created in 1993 • Original report – IL-10 KO phenotype • Chronic enterocolitis in mice housed under conventional conditions • Colitis affecting only the proximal colon in mice housed under SPF conditions • Model for human idiopathic inflammatory bowel diseases? • Real cause: normal resident enteric bacteria especially in combination with Helicobacter hepaticus Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  16. A8-023287 • Differential diagnoses • Citrobacter rodentium infection • Young mice or certain strains of mice affected (e.g. FVB) • Sticky, unformed feces • Rectal prolapse often occurs • Brush border of the mucosa of the affected bowel is carpeted with attached cocco-bacilli • Descending colon is affected • Hyperplastic colonic mucosa with excessive numbers of goblet cells and leukocytic inflammation in the lamina propria Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  17. A8-023287 • Differential diagnoses – continued • Coliform typhlocolitis • Atypical, non-lactose fermenting E. coli • Intestinal lesions only occur in triple deficient mice (devoid of T, B, and NK cells) • Segmental colonic hyperplasia with erosions and mononuclear inflammation • Helicobacter sp. induced proliferative typhlocolitis • Immunodeficient (SCID & nude) mice Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  18. A8-023287 • Differential diagnoses – continued • Enterotropic MHV • Neonatal mice in naïve mouse colonies • Immunodeficient (SCID) mice • Attenuated villi with syncytia formation (balloon cells) Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

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