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The majority of freshly sorted SIV-specific CD8 + T cells cannot suppress viral replication in SIV-infected macrophages. Lara Vojnov, PhD Department of Pathology and Laboratory Medicine University of Wisconsin-Madison Madison, WI, USA. Evidence of HIV/SIV infection of macrophages.
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The majority of freshly sorted SIV-specific CD8+ T cells cannot suppress viral replication in SIV-infected macrophages • Lara Vojnov, PhD • Department of Pathology and Laboratory Medicine • University of Wisconsin-Madison • Madison, WI, USA
Evidence of HIV/SIV infection of macrophages In vivo SIVmac239 infection of macrophages in intestinal lymphoid nodule CD68+ macrophages comprise 10% of the SIVmac251 cell population in vivo Veazey et al. Science 1998 Zhang et al. Science 1999
Evidence of HIV/SIV infection of macrophages PNAS 2001 Veazey et al. Science 1998 Zhang et al. Science 1999
Hypothesis Macrophages play an important role in HIV/SIV infection as a viral reservoir resistant to CTL-mediated lysis.
SIV-specific CD8+ T cells suppress viral replication in SIV-infected CD4+ T cells MHC matched infected targets MHC mismatched infected targets
SIV-specific CD8+ T cells poorly suppress viral replication in SIV-infected macrophages MHC matched infected targets MHC mismatched infected targets
SIV-specific CD8+ T cells poorly suppress viral replication in SIV-infected macrophages 32% 88%
SIV-specific CD8+ T cells poorly suppress viral replication in SIV-infected macrophages 30% 3%
Bulk CD8+ T cells suppress viral replication in SIV-infected CD4+ T cells
Bulk CD8+ T cells poorly suppress viral replication in SIV-infected macrophages
Acknowledgements National Institutes of Health Jorge Almeida Danny Douek NEPRC R. Paul Johnson FIOCRUZ – Rio De Janeiro Myrna Bonaldo Marlon Veloso de Santana Patricia Costa Neves Ricardo Galler University of Wisconsin-Madison Mauricio Martins Alexander Bean Jonah Sacha Holly Kolar Nancy Wilson Benjamin Burwitz David O’Connor University of Miami David Watkins Ann Dauphin Mario Stevenson