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Dr. Nurit Haspel Bioinformatist. Proteins in Reproductive Systems How do proteins get cut? What proteins does the digested pieces interact with after?. Tris : Keeps the DNA soluble in water Boric Acid: Is a weak acid EDTA: binds metal cations, such as Mg 2+.
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Dr. NuritHaspelBioinformatist Proteins in Reproductive Systems How do proteins get cut? What proteins does the digested pieces interact with after?
Tris: Keeps the DNA soluble in water • Boric Acid: Is a weak acid • EDTA: binds metal cations, such as Mg2+ Ingredients of TBE and their Functions
10.8g Tris • 5.5g Boric Acid • 0.93g EDTA PREPARATION OF 100ML OF 10X TBE
C1V1= C2V2 • (10x)(?)= (500ml)(1x) • ?=50ml of 10x TBE stock • 440 ml of MQ to make 500ml PREPARATION OF 500ML 1X TBE FROM 10X STOCK