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REPORTS in PDF format Due 23.59h, Tuesday December 11th. Grading Key for final report. Correct format (order/spacing/legends/etc.) Correct methods Basic results Appropriate references Discussion (analysis). 30% 20% 20% 15% 15%. 25% of final grade for 446L (100/400)
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REPORTS in PDF format • Due 23.59h, Tuesday December 11th Grading Key for final report • Correct format (order/spacing/legends/etc.) • Correct methods • Basic results • Appropriate references • Discussion (analysis) 30% 20% 20% 15% 15% • 25% of final grade for 446L (100/400) • 20% of final grade for 546L (100/500)
LOCUS Echinostomatidae_EPS4 14426 bp DNA circular 06-DEC-2018 DEFINITION Uncharacterized Echinostomatidae isolate PE4 mitochondrion, complete genome. ACCESSION Echinostomatidae_EPS4 VERSION KEYWORDS . SOURCE mitochondrion Uncharacterized Echinostomatidae ORGANISM Uncharacterized Echinostomatidae Unclassified. REFERENCE 1 (bases 1 to 14426) AUTHORS Adema,C.M. TITLE An uncharacterized physid snail from NM JOURNAL unpublished REFERENCE 2 (bases 1 to 14426) AUTHORS Robinson,A.J., Bennett,A.E., Britton,T.L., Buser,A., Campbell,M.L., Carroll,N.D., Chavez Trujillo,D.G., Cutillas,V., Das,P.K., Dugan,D.M., Graf,K.E., Jeshurin,M., Johnson,J.M., Locke,J.W., Marlenee,J.F., McCready,J.E., Medley,J.J., Potts,Z.L., Ruiz,R.E., Wright,K.T., Zlatkin,K., Bu,L., Natvig,D.O. and Adema,C.M. TITLE Direct Submission JOURNAL Submitted (06-DEC-2018) Biology, university of New Mexico, 2428 Castetter Hall University of New Mexico, Albuquerque, NM 87131, US COMMENT Bankit Comment: TAX: Yes, new species/combinations; SEE ATTACHMENT Bankit Comment: TOTAL # OF SEQS:1 Bankit Comment: SUBMITTER_TAX_REPLY: Yes, new species/combinations; parasite observed as echinostome-type cercaria, shed from physid snail, mitogenome sequence most similar to mitogenomes of echinostomatidae ##Assembly-Data-START## Assembly Method :: mitobim v. 1.8 Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..14426 /organism="Uncharacterized Echinostomatidae" /organelle="mitochondrion" /mol_type="genomic DNA" /isolate="PE4" /country="USA: Albuquerque NM" /lat_lon="35.2144 N 106.596 W" /collection_date="22-Jul-2016" /note="Lineage: Eukaryota; Metazoa; Platyhelminthes; Trematoda; Digenea; Plagiorchiida; Echinostomata; Echinostomatoidea; Echinostomatidae; echinostome-type parasite patent infection in physid snail" gene 1..1539 /gene="cox1" CDS 1..1539 /gene="cox1" /note="[intronless gene]" /codon_start=1 /transl_table=9 /product="cytochrome c oxidase subunit I" /translation="MYKWGWLFTLDHKRVGLIYMVIGLWGGFLGLSLSILIRLNYLDP YDNLVSPEIYNYVVTSHGIVMVFFFLMPVLIGGFGNYLLPILLGVPDLNLPRINALSA WLLLPAAVCLGLSMLGGAGVGWTFYPPLSTSTYSGWGVDFLMFSLHLAGVSSILGSIN FICTILEGLLEEGTGQFSIIIWAYLFTSILLLVSLPVLAAAITMLLFDRSFGSAFFDP LGGGDPVLFQHLFWFFGHPEVYVLILPGFGIISHICMTLTNNDSLFGYYGLILAMAAI VGLGSVVWAHHMFMVGLDVETAVFFSSVTMVIGIPTGIKVFSWLIMLGGTRGRLWDPV VWWIIGFIVLFTIGGVTGIMLSASILDTLLHDTWFVVAHFHYVLSLGSYSSVIISFIW WWPVITGYSLNYGMLWAHWGVSMVGFNLCFFPMHYLGMCGLPRRVCVYDPDLYWLNLC ASSGGIISGISAFFFVFILWESMLVRNRVASSWGSNSLVLNVVTLPTPHHMLYSVGGD RWLF*" tRNA 1570..1643 /product="tRNA-Thr" /anticodon=(pos:1609..1611,aa:Thr,seq:tgt) rRNA 1644..2620 /product="16S ribosomal RNA" tRNA 2630..2694 /product="tRNA-Cys" /anticodon=(pos:2660..2662,aa:Cys,seq:gca) rRNA 2695..3439 /product="12S ribosomal RNA" gene 3440..4063 /gene="cox2" CDS 3440..4063 /gene="cox2" /note="[intronless gene]" /codon_start=1 /transl_table=9 /product="cytochrome c oxidase subunit II" /translation="MVFSLNVLYLDLISYIVIVCSFIPVWVFTVLGWQLFSSETIASL ENESDAAEFLWTVFPTGAVGFLCYYNLGCIDYDIVDIGCKTIKVVGRQWYWSYHCDYY GGGEYDSVMSDFVIGVDKPLRMVANDCHRFLVTSSDVIHSFSIPQFNVKLDAIPGRLN FTTYTPSHFGIFIGYCTELCGAGHAYMPIVVEVIPDTLVPEEGVAGY*" gene 4073..4525 /gene="nad6" CDS 4073..4525 /gene="nad6" /note="[intronless gene]" /codon_start=1 /transl_table=9 /product="NADH dehydrogenase subunit 6" /translation="MIYSLLAGVYFSCLLGFSLVSHPVVYCVLLLSSALSVAGLVYLV VGFSWYLAIFCLVYIGGVYVLFIFISIHSPNPVANVGGSSFIPIIMLGLFFCILIHSF SPLPAGLDSSNYLCSYFEGFSYCLFCLVLMVGFVSISVVVGSKGSFFR*" tRNA 4525..4590 /product="tRNA-Tyr" /anticodon=(pos:4557..4559,aa:Tyr,seq:gta) tRNA 4591..4655 /product="tRNA-Leu" /anticodon=(pos:4621..4623,aa:Leu,seq:tag) tRNA 4725..4786 /product="tRNA-Leu" /anticodon=(pos:4755..4757,aa:Leu,seq:taa) tRNA 4788..4851 /product="tRNA-Arg" /anticodon=(pos:4817..4819,aa:Arg,seq:tcg) gene 4852..6417 /gene="nad5" CDS 4852..6417 /gene="nad5" /note="[intronless gene]" /codon_start=1 /transl_table=9 /product="NADH dehydrogenase subunit 5" /translation="MLLVSLFILGLVMWWLQSVGGIAGYFCWLSYVSFPVFGFMLDSV SILCLYMLVCCGFVALFYCFHYFGGSSDASSLFPLVVWFLGVMAILVLSSSMLFSLIF WEYLGLVSFFLILFYSNMSSLRASLITLFASRFGDVSLFVVIMWLSCCWDLSLSVFLF LYMLVVMTKSACYPFVSWLLEAMRAPTPVSSLVHSSTLVAAGVWFVLRYSYLGVGGLY YWLGGFCFVTIFLTSFAALVFMDLKKIVALSTCNNVSWCILFFVFGDLYLSLLQLISH GVCKCYLFMSVGDLMGQSGSSQSSVGVYLGRYSGVFLPVIQGFLVLSLCGLPYIGVFF SKHVLFSGLLYSGGLGVFVLSLVCLFLSYVYSFRFVFLLLGSMGGLSSGYSSFFFIIC PLVILGTLVNYFGSLLLIEDYALGGFYSSLILCVQFLGCFIGGYLYFSLMGCGRWFSM LSGCEGYVNSFYSGFVNMSSLCVVSFYRWEVYLMGVLTDFSFRGWLLRSSFFSLNFVV LGLFFIFVFCYVV*" tRNA 6427..6492 /product="tRNA-Gly" /anticodon=(pos:6459..6461,aa:Gly,seq:tcc) tRNA 6993..7061 /product="tRNA-Glu" /anticodon=(pos:7022..7024,aa:Glu,seq:ttc) gene 7540..8169 /gene="cox3" CDS 7540..8169 /gene="cox3" /note="[intronless gene]" /codon_start=1 /transl_table=9 /product="cytochrome c oxidase subunit III" /translation="MYNAWVVMVGILSLFLWQLWGVLFFLAISLVSLWFLAKESLYTA KHFATGFWIFIASEVVAFGSLFVICVNSEEKVVNPISHFMELPLLGCFLLSGSSITIT TYHHYYGTAGCHYYLLGTIILGWCFILLQVYEFYDCCCDITFCFYIGVCFATVGLHFL HVMGGLIVMMVLYCCGDEGVPQSNIDFVVWYWHFVDYVWLFVFLIIYIS*" tRNA 8172..8239 /product="tRNA-His" /anticodon=(pos:8207..8209,aa:His,seq:gtg) gene 8240..9349 /gene="cyb" CDS 8240..9349 /gene="cyb" /note="[intronless gene]" /codon_start=1 /transl_table=9 /product="cytochrome b" /translation="MLSLVRTNVFDLPTNLSLSYFWCGGFMISSFLVIQVVSGIILSF LYVADSYMSFGCVLDFTSEGLFVWLVRYFHIWGVTFIFLLFFVHMGRALYYSSYSKLG VWNVGFILYLLMMAEAFLGYILPWHQMSYWAATVLTSILNSIPLIGGVLYKFVVGGFS VTNVTLVRVFSAHVCLAFIILGFSVVHLFYLHKGGSNNPLFAPSGYGDVVLFHSFFTN KDGFVLFILLFACSALLLLCPDLVLDIESYIQADPLVTPVSIKPEWYFLAFYAMLRSV ESKVGGLVLVLVFLLLLWLPTLNKSCAYSLGRQYVFWFAVFNFILLSYLGACHPEAPF IIISKFSSFSIVFLLMMFKGLWVVPYSGGFPRMGL*" gene 9355..9627 /gene="nad4L" CDS 9355..9627 /gene="nad4L" /note="[intronless gene]" /codon_start=1 /transl_table=9 /product="NADH dehydrogenase subunit 4L" /translation="MLGSGSFVLYLGSLIVLFSFFMSLSRLLNCLIVVENFNVLLLLW CLLGQWDEFRMLFIALIVIFTVEVTLGLVVLTRLWDSSSLIGIVGE*" gene 9630..10874 /gene="nad4" CDS 9630..10874 /gene="nad4" /note="[intronless gene]" /codon_start=1 /transl_table=9 /product="NADH dehydrogenase subunit 4" /translation="MGSSVFLGVSVLGLSFLDLGSLGLYSVEFGGLFIFDSVSFYLSV LSLFLACSLVMVGGSSLSLMSCIMLSVSVVSSVLCYSCINGLGFWVFYEMSILPLLFL LISESPYSERFIASWYLLGYVVVSSLPMLLCIFYLGGVCGGYDFQLWVSEYSLGSGFL VYLILSVMFITKIPLPPFHVWLPIVHAEASSPVSMCLSGYIMKLGLLGVCRFSCSLLS DYIFSSYYVLISLCFAVLFFFSAARELDGKRWLAFLSLSHIVIVSLCFNVCSYDSVLL SFLYCLGHGLSAGVTFLLLWMIYDISGTRNWVILKGALSGSLLLRCLAVSCVSSAASI PPTICFFSEVVILSEGGFVSMLFALMMSLYLFVGGLVPLFLVGCLLSRHYSISFGSGY VSSYIAGVWFLVVWCFLLFLVF*" tRNA 10882..10948 /product="tRNA-Gln" /anticodon=(pos:10916..10918,aa:Gln,seq:ttg) tRNA 10965..11028 /product="tRNA-Phe" /anticodon=(pos:10995..10997,aa:Phe,seq:gaa) tRNA 11030..11099 /product="tRNA-Met" /anticodon=(pos:11067..11069,aa:Met,seq:cat) gene 11103..11621 /gene="atp6" CDS 11103..11621 /gene="atp6" /note="[intronless gene]" /codon_start=1 /transl_table=9 /product="ATP synthase F0 subunit 6" /translation="MLVTRLNAIFSAVSSLVTGGWGDSYYRYCLFGLLVSFLLLRIPY IYGMQGFALFIILVVFPLFMSLLLSRVVDVGPTSFFSGFVPPGTPLWIAPFVCLAETL SYIVRPIVLMIRPFVNLTIGTLGGVAIGGLGFKFGVWVISFLFLLFFYEVFVALVHWF IVCNILSFSEDH*" gene 11631..12497 /gene="nad2" CDS 11631..12497 /gene="nad2" /note="[intronless gene]; [intronless gene]" /codon_start=1 /transl_table=9 /product="NADH dehydrogenase subunit 2" /translation="MRGLIISLFGIVGVFCFSVLMFSSNNLSFFWLFLELATLCAIPL FFLSGEGGVLVGLFNYIVISSISSSLMLCGILSGELLALLVVGLLLKFGLFPFWGWVY KVGLGSNWLVLWVVSTFLKSPVFLFPFFLSCGGYSLVNYVCCITFLGLSVLFWLYSLN WYACWCHMMLSSSAALVAMSGVLAADVLAAIFFVYCVWCSLVVVFFSFYEYDFLGGLG YYFWFCFLLISMPVSISIFYKLVMAVGIYSCWFVVLCCWVIYSVSEQFYLLKFVMSFS LPKASGWFVSEV*" tRNA 12501..12565 /product="tRNA-Val" /anticodon=(pos:12532..12534,aa:Val,seq:tac) tRNA 12593..12655 /product="tRNA-Ala" /anticodon=(pos:12625..12627,aa:Ala,seq:tgc) tRNA 12663..12728 /product="tRNA-Asp" /anticodon=(pos:12694..12696,aa:Asp,seq:gtc) gene 12774..13631 /gene="nad1" CDS 12774..13631 /gene="nad1" /note="[intronless gene]" /codon_start=1 /transl_table=9 /product="NADH dehydrogenase subunit 1" /translation="MAFVIVMVFVAFFILGERKVLGYMQIRKGPNKVGLFGLLQSFAD LLKLVIKFKVMFFQNRTWLSWLGVYLLVFLSCGYCVVLALAYSGISSGYLMLWFLVLT SITGYSLLSVGWGSHNKFALLSCVRSAFGSVSFEACFMCVVIMVAIVVGTYGVASLVT SAWLINLVLPLVYGLWLVGILCECNRTPLDYAEAESELVSGLNTEYCNVPFTCLFACE YLIMVIFSWFSSVLFWGGSLIIGLTLFHIMFFVWARATLPRVRYDYFVSFMWRCAILV LVFSFFFII*" tRNA 13642..13711 /product="tRNA-Asn" /anticodon=(pos:13680..13682,aa:Asn,seq:gtt) tRNA 13717..13784 /product="tRNA-Pro" /anticodon=(pos:13749..13751,aa:Pro,seq:tgg) tRNA 13785..13849 /product="tRNA-Ile" /anticodon=(pos:13814..13816,aa:Ile,seq:gat) tRNA 13858..13929 /product="tRNA-Lys" /anticodon=(pos:13892..13894,aa:Lys,seq:ctt) gene 13940..14287 /gene="nad3" CDS 13940..14287 /gene="nad3" /note="[intronless gene]" /codon_start=1 /transl_table=9 /product="NADH dehydrogenase subunit 3" /translation="MFSLCILFFLVFFMVVVFHLFVWNTDLGIFSGERSWVSSFECGF IAQRLVENYFSYTYFILLVFFVVFDLEVSLLLNLPLQGILYKNLSYYLFFLVLLAVGF GVEISRGYIRWSY*" tRNA 14291..14349 /product="tRNA-Ser" /anticodon=(pos:14312..14314,aa:Ser,seq:gct) tRNA 14359..14423 /product="tRNA-Trp" /anticodon=(pos:14390..14392,aa:Trp,seq:tca) BASE COUNT 2831 a 1596 c 3791 g 6208 t ORIGIN 1 gtgtataagt gaggttggtt atttacgttg gatcataagc gtgttggttt aatttatatg 61 gttattggtt tatgaggggg gtttttgggt ttgtctttaa gtattttgat tcgcttgaat 121 tatttggatc cttatgataa tcttgtttct ccagagattt ataaatatgt ggttactaga 181 catgggattg ttatggtgtt ctttttttta atgccagtat tgattggggg gtttgggaat 241 tatttgttgc ctattttatt gggtgttccg gatttaaatt taccccgcat taatgccttg 301 agtgcgtggc tcttattacc tgctgctgtt tgtctaggtt tgagtatgtt aggtggtgct 361 ggagtaggtt ggacctttta tcctcccctg tctactagaa cttatagggg atggggtgtt 421 gattttttaa tgttttcttt acatttggcg ggtgtttcaa gtatattagg ttcaattaat 481 tttatttgta ctatattgga ggggttatta gaagaaggta cgggtcagtt tagtattatt 541 atttgggctt atttgtttac ttctattttg cttttggttt ctttgcctgt attggctgca 601 gcgataacta tgttgttatt tgatcgtagt tttggttctg ccttttttga tccgttggga 661 gggggagacc ctgttttatt tcagcattta ttttgatttt ttgggcatcc ggaggtttat 721 gttttgattt tgccagggtt tgggattatt agacatattt gtatgacttt aacaaataat 781 gattctcttt ttgggtacta tggtttaatt ttggctatgg cagctattgt gggtttaggt 841 agcgtagttt gagcacatca tatgtttatg gtcggtctgg atgtggagac tgctgtgttt 901 tttagttctg ttactatggt tattggaata cccacaggaa ttaaggtttt ttcttgatta 961 attatgttgg gtggtactcg ggggcgttta tgggatcctg tggtttgatg aattataggt 1021 tttatagttt tgtttactat tgggggagta accggtataa tgctttctgc ctctattctg 1081 gataccctat tgcatgatac atgattcgtg gtggcgcatt ttcattatgt cctttcatta 1141 ggttcctata gtagggtgat tatttcattt atttggtggt ggcctgtaat tactggttat 1201 agcttaaatt acggtatgtt gtgggctcat tggggtgttt caatggtggg attcaatctt 1261 tgtttctttc ctatgcatta cttgggtatg tgtggtttac cccgtcgtgt gtgtgtttat 1321 gacccggatc tttattgatt aaatctttgt gcaaggtctg gaggtataat ttctggtatt 1381 agtgccttct tttttgtttt tatattgtgg gagtctatgc tggttcgtaa tcgggtggct 1441 tcttcttgag gaagaaactc gttggtttta aatgtagtta ctctaccaac acctcaccac 1501 atgctttata gtgttggtgg ggatcgttgg cttttttagg tgtgcgcttg gtatagcttt 1561 tttataaatg gggggttagt ttatgtgttg ttgctagaat actgcttttg taaagcaggg 1621 gggttgtttt gtgacacctt ccacgaatat aatttagaga ttgtaagtgt tgcagtttag 1681 gctatttgag gtaccttttg catcatgatt tattgaaggt ctttgctgtt ttgtattgac 1741 ccgaaaaatg ccgatttctg tctttacagt ttggtactca gtctaggacg ggtagtttag 1801 gttagataaa gattagggtt gtgagaaata attcggggca tttaatatct ggttctgggg 1861 gttaatatag atttagtttt ctggcaagga tgtcggggaa cggttaatag catgtttact 1921 ggtagtgatt tgcgtgggtt aaagttaccc ctgcatagtg attgtgttaa gactctctgg 1981 tttaggtgtt atgctttagt ggtttacccc agtattgatt aattttagta aagtatgata 2041 aaataagtag gttttgatta atttcatttt ttctcgattt tctccggtct gtttattaaa 2101 aacatttcca tttagagttt tgaatggtag tgcctgccca gtgcttaatt gtaaatggcc 2161 gcagtatttt gactgtgcta aggtagcata attagttgcc ccataattgg gggattgttt 2221 gaaaggtttg accagggggg tgtgtattag attgaaatta agtctgaaat taagttgctg 2281 gtgcagactc cagcagaagt ttattagacg gaaagacccc gagatcttta tattgcgatg 2341 tttacttggg gcaaggccat atagtttatg tggttttaga tcctaaggga tagaaggttt 2401 aagttacctc ggggataact aggtaaaaaa tgaggagaga tcttatcgat tcgtttgatt 2461 gccatctcga tgttgactta ggaaatgtgg tgaggtgcag aagtttcatt acgtggtctg 2521 ttcgaccctt tgttccttca tgagttgagt taagaccggc gtgagccagg tcggttctta 2581 tctataattg gtgtgaacta gtacgaaagg attgtttgca ttttatgttg ggcgtgacgc 2641 tggtttagcg tgatactctg caaaggtatt agagatatta agtatcctcg tcttatctat 2701 ttaattctgg ttgaagaaga gggggaggca ggtctacata aaagaggttt atggtctcaa 2761 cagaggatgt cgttgttgag gtttagtagc tagatcttag gttttggagt aatccagatt 2821 aggttgcctc tcgaaaagtg gtgaatactc agtgccagca tccgcggtta ttctgttagc 2881 tttgtcttct gcgataggtt aaaatggttt aagagttagt ggccagtaag gggtagaatc 2941 tttctggttt tgaaattggc tcttaggcgt tttgatgtca cgataaatta aggggattag 3001 atacccccgt aatggtgttt aagttttgtt ccttagtata aactaaaagg atttggcagc 3061 cgacgaggtt cttccggggg agcgtgtatc ataagagata gtccactcaa gaatcaaccc 3121 cctttagctt agttagtgta tatccggttt aaaatttgtg gttaatgtct atgagtgtaa 3181 ctatttgttt aatccaggtc catgtgctgc ttatattggg gggtttatgc gctacagttg 3241 taaagggata gataggaagg ttatttatca ggtttaggac tcggaagtag gcgttatgta 3301 gaattagtat gttgtgtcga agctgattta gttggggtac acaccgcccg tcacccaggg 3361 cgatagctgt ggtaagtcgt aacatggtaa tgctagggga acctggtgtt agttggttta 3421 ttgctgtatg caagaatcta tggttttttc tttgaatgta ctttatttgg atttgattag 3481 ttacatagtc atagtctgta gatttatacc ggtttgggtt tttactgtgt tgggctggca 3541 gttgtttagg tctgagacga tagcgtctct tgaaaatgag agggatgctg cggagtttct 3601 ttggactgta tttccaaccg gagcggttgg gtttttgtgt tattataatt tgggttgtat 3661 tgattatgat attgtggata ttggttgtaa gacgattaag gtggtaggcc ggcagtggta 3721 ctgatcttat cattgtgatt actatggtgg gggggagtat gattctgtga tgagtgactt 3781 tgttattggg gttgataagc ctcttcgaat ggttgctaaa gattgtcatc ggtttttagt 3841 gacttcttcg gatgtaattc actcattttc tattccacaa tttaatgtga agcttgatgc 3901 gattccaggt cgtttgaatt ttacgactta tacacccaga cattttggta tttttattgg 3961 ttattgtacg gagttatgtg gtgcgggtca cgcgtatatg cctattgttg tggaggttat 4021 acccgatact ctggttccag aggagggtgt agcgggttat tagtttgaat aggtgattta 4081 ttcattattg gcgggtgttt actttagttg tttgttaggg ttttctttgg tatctcaccc 4141 tgttgtttat tgtgttttat tattatcttc tgctttaagg gttgctgggt tggtttattt 4201 ggttgttggg ttttcttggt atttggctat attttgttta gtttatatag ggggggttta 4261 cgtgttgttt atttttattt ctattcacag accaaacccg gtagctaaag ttgggggtag 4321 tagttttatc cccataatta tgctgggttt gtttttttgt attctcatcc attcattttc 4381 cccccttcct gcgggtttag atagcaggaa ttatctttgt tcttattttg aggggttctc 4441 ctattgttta ttttgtttgg tgttgatggt tggattcgtc aggattagag tggttgtagg 4501 gagtaagggt tcgttttttc gttagtcggt ttagcatatg gttagtgtgt gggattgtag 4561 ctcccgagga ggattaattt ctggccggaa tttggagtgc cagattgaat gggtatgatt 4621 taggattata ttatggtttg ttggaccctc tagcgtgtaa gattttatcg ggtttgattt 4681 gaagtcattc ttttcatatt aggttatgat gctcgcttga tgatacatga gtgccagatt 4741 atatgggttg gttttaagca tcaaatatgg ggtttcccct cgtgtatttt gatatccctt 4801 taatgggggt tacgtttcgg ccgtaattgt gggaaagttt ctctatcaaa ggtgttgttg 4861 gtatcgttgt ttattttagg tttggtgatg tggtggcttc agagtgtggg gggcattgcc 4921 gggtattttt gctggttgag ttatgtaagc tttcctgttt ttgggtttat gcttgattcg 4981 gttagtatac tttgtttata catgctagtt tgttgtggtt ttgtggcgtt attttattgt 5041 tttcattatt ttgggggttc tagggatgcg agtaggttgt ttcccctagt tgtttggttt 5101 ttgggggtta tggcaatttt ggtgctttct tcttctatgc tgttttcttt gattttttga 5161 gaatatttgg ggttggttag gttttttttg attttgtttt attctaaaat gagtagtttg 5221 cgtgcttctt tgattacttt gtttgcttct cgttttgggg atgtgtcttt gtttgttgtg 5281 ataatgtggt tatcttgttg ttgggattta tcgttatctg tttttttgtt tctttatatg 5341 ttagtggtaa tgactaagag ggcttgttat ccgtttgtat cttggttgct tgaggctatg 5401 cgggctccca cgcctgttag ttctttagtt cattcttcta ctcttgtagc ggcaggggtt 5461 tggtttgttt tgcgttattc gtatttaggt gttggggggt tatattattg gttggggggg 5521 ttttgttttg ttactatatt tttaacttct tttgctgctt tggtttttat ggatttaaag 5581 aagattgtag ccctttctac gtgtaataat gtttcttggt gtattctgtt ttttgttttt 5641 ggtgatttat atttgtcttt gttacagctt ataagtcatg gggtgtgtaa gtgttatttg 5701 tttatgagag tgggggattt gatggggcag tccgggtcaa gtcaaagttc agtaggtgta 5761 tatttaggac gttactctgg tgtgtttttg cctgtaattc aggggttttt agtattatct 5821 ctttgtggtt tgccttatat aggtgtgttt tttaggaagc atgttttgtt ttctgggttg 5881 ttgtatagtg gtgggttggg tgtgtttgtg ttgtccctag tttgtttgtt tttgtcttat 5941 gtttattctt ttcggtttgt gtttttgtta ttggggagta tgggcggttt gagaagtggt 6001 tattcaagat ttttttttat aatatgtccc ctggtgattt taggaacctt ggttaaatat 6061 tttggtaggt tgttgctgat tgaagattat gcgttggggg gtttttattc tagtttgatt 6121 ctgtgtgtac aatttttagg gtgttttatt gggggttatt tatattttag gttgatgggg 6181 tgtggtcgtt gattttctat gttgagtggc tgtgaggggt atgttaatag tttttataga 6241 ggttttgtga atatgtcaag tttgtgtgta gtttcttttt atcgttggga ggtatattta 6301 atgggtgtgt tgactgattt taggtttcgg ggttgattgt tgcgtagttc ctttttttcg 6361 ttaaactttg tagtgttagg gttgtttttt atatttgttt tttgttatgt tgtttagggt 6421 gtgtggacgt caatagtata tgtaaagtat gccgtctttc caagtcgggg gtctagcttg 6481 ttagttgtcg tattgttggg ggagaggata gtaattgggt ttagggggta gggttaaaaa 6541 tatttggagt tgggcatcaa gttattgttt ccttgataga tatattatat taatgtttat 6601 aatgttgcag attttctgtt aaaggtttgt aagtattttc aggttagaat ttttgttatt 6661 ttaaaaaatt ttcttttgta aaactttcct caattttggt ttctctctcg gagtaaagtg 6721 ttaaatttat ctggtgagat taccttgtaa atttaggttt gtaatacccc ccccttacaa 6781 gattttttgt ttggggggta gggttaaaaa tatttggagt tgggcatcaa gttattgttt 6841 ccttgataga tatatagtaa aaaacggtgg aggcgggcag ggacaatgcc atagagtaaa 6901 atattcaggt ggaggggagg tttatgttcg gcctcaagtc gggggtctag cttgttagtt 6961 gtcgtattgt tgggggagag gatagtaatt gggtcgatta ggtgttgtta catgctagtt 7021 tttcgtactg gagtggatct atattttggg attaatcgat gtattggttt ttaaattaat 7081 ggttgggtaa tgtaagtatt ttcaggttag aatttttgtt attttaaaaa tttttctttt 7141 gtaaaacttt cctcaatttt ggtttctctc tcggagtaaa gtgttaaatt tatctggtga 7201 gattaccttg taaatttagg tttgtaatac ccccccctta caagattttt tgtttggggg 7261 gtagggttaa aaatatttgg agttgggcat caagttattg tttccttgat agatatatta 7321 tattaatgct tataatgttg cagattttct gttaaaggtt tgtaagtatt ttcaggttag 7381 gtttaggtta aaggtataat tggcatctgc tgtaaggcaa tgcccccggg agggggagct 7441 gatgggtagt aaggttaggt taaaatagat gtataatctg cgggcagtgg gaggtttgtg 7501 ctaccttctt ggatttgatt agagatgagt tggttgccaa tgtataaagc ttgggttgta 7561 atggtgggta ttttgaggtt gtttttgtgg cagttatggg gcgtgttatt ttttctggct 7621 atttctttgg tttctttatg gtttttagct aaggaatctt tgtatacggc caagcatttt 7681 gctacggggt tttggatatt tatagctagc gaggttgtgg cctttggtag cttgtttgtt 7741 atatgtgtta atagggagga gaaggttgtt aatcctattt cacattttat ggagcttccg 7801 ttgttggggt gttttctttt aaggggttct tcaataacaa tcactaccta tcatcattat 7861 tatggtacag ccggctgtca ttattatttg ttgggtacta taattttggg ttggtgtttt 7921 attctcttac aggtttatga gttctatgat tgttgttgtg atattacttt ttgtttttat 7981 ataggggtat gttttgcgac ggttggttta cattttcttc acgttatggg cgggttaatc 8041 gtaatgatgg ttttatattg ttgtggtgat gagggtgtcc cacagtcaaa tattgatttt 8101 gtggtttgat actgacattt tgtagattat gtttggttat tcgttttttt gataatttat 8161 atttcttaaa ttctcttgta ggttatggta cttaaacctc cgatttgtgg tgtcggcaaa 8221 tcctgaacgg acgaaagaaa tgttgtcttt ggttcgaaca aaagtatttg atctaccaac 8281 aaatctatct ttaagctatt tttggtgtgg tgggtttatg attaggagtt ttttggttat 8341 tcaggttgtt tctggtataa ttctgtcttt tttgtatgtt gctgattctt atatgagttt 8401 tgggtgtgtt ttagatttta cttctgaggg tttgtttgta tggctcgttc gttattttca 8461 tatttggggt gtaaccttta tattcttgtt gttttttgtt catatgggtc gtgctttata 8521 ttattcgagg tataggaagt tgggggtttg aaatgtgggg ttcattttat atttgttgat 8581 gatggcggag gcttttttgg gttatatttt gccttgacat caaatgtctt attgggctgc 8641 tactgtttta acttctattt taaatagtat ccctttgata gggggtgttc tttataagtt 8701 tgttgttggg gggttctctg taactaatgt gacgttagtt cgtgtatttt cggctcatgt 8761 gtgcttagct tttataattt tgggttttag tgttgtgcat ttattctatt tacataaggg 8821 gggttccaat aacccgcttt ttgctccaag gggttatggt gatgtggtgt tgtttcattc 8881 cttttttact aataaggatg gttttgtgtt gtttatatta ttatttgctt gtagtgcttt 8941 attgttgctg tgtccggatt tagtgctgga tatagagaga tatatacaag cggatccttt 9001 ggtgactcct gtttctataa agccagagtg atatttttta gctttttatg ccatgcttcg 9061 ttctgtggag tccaaggttg gggggttagt tttggtttta gtattcttgt tgttgttatg 9121 attgcctact ttaaacaagt cttgtgctta tagtttgggt cgtcagtatg ttttttggtt 9181 tgctgttttt aattttattt tgttaagtta tttgggggcg tgccatccag aggctccatt 9241 tattataatt agtaagtttt ctaggttttc aatcgtgttt ttattaatga tgtttaaggg 9301 gctgtgggtg gttccttatt ctgggggttt tcctcgaatg ggtttatagt tagtatgctt 9361 gggaggggga gctttgtttt atatctgggt tctttgattg tgttgtttag tttttttatg 9421 tctttatctc gtctgttgaa ctgcttaatt gtagtggaga aatttaaagt attgttgctg 9481 ttatggtgtt tattggggca gtgggatgag tttcgaatgt tgtttattgc tttaatagta 9541 atttttactg ttgaggtgac tttaggattg gtggttttaa cgcgattgtg ggattcaaga 9601 agtttgattg gtatagttgg ggagtaatcg tgggttctag tgtatttcta ggagtgtcgg 9661 ttttaggtct gaggttcttg gatttaggtt ctttgggatt atattctgtt gagtttgggg 9721 ggttatttat atttgattca gtaagttttt atttgagggt tctttcatta ttcttagctt 9781 gttcacttgt aatggtgggt ggtagtaggt tgagtcttat gtcctgtatt atgttgtctg 9841 ttagtgttgt tagttctgtt ttgtgttatt cttgtattaa aggtttaggg ttttgagtct 9901 tttatgaaat gtcaatactt ccgctgttgt ttctattaat atcggagtct ccctattctg 9961 agcgatttat tgcttcctgg tatttattgg gttatgttgt ggtcagtagc ttgccaatgt 10021 tattatgcat tttttatttg ggtggagtgt gtggtggtta tgattttcag ttgtgagttt 10081 cagaatatag tttaggtagt ggatttttag tttatttgat tctttctgta atgtttataa 10141 ctaagattcc acttcctccc tttcatgtgt ggttaccaat tgtgcatgcg gaagctagga 10201 gacctgtttc aatgtgtttg agggggtata taatgaagtt gggtttattg ggtgtttgtc 10261 gatttagttg tagtttgttg tcagactata tttttagtag ttattatgta ttaattagat 10321 tatgttttgc agttttattt ttttttagtg cagctcggga gttagatggt aagcgttggt 10381 tggccttttt gaggttgtct cacattgtta tagtttcttt gtgttttaat gtttgttctt 10441 atgatagtgt tctgttgtct tttttgtatt gtttgggaca tgggttatct gcaggtgtaa 10501 cttttttgct actctggatg atatatgata tttcgggaac tcgaaattgg gtaatattga 10561 agggggcttt atctggcagg ttactgttac gttgtttggc agtatcatgt gtttctaggg 10621 ctgcgtctat acctcccacg atatgcttct tttctgaagt ggtgatatta agggagggtg 10681 gatttgttag aatgctattt gctttgatga tgagtctgta tctattcgtt ggggggctag 10741 ttccgttgtt tttggtgggt tgtttactat cacgtcatta ttcaatcagg tttggaaggg 10801 gttatgtgtc gaggtatatt gcgggtgttt gatttttagt ggtttggtgt tttttactct 10861 ttttggtgtt ttagtgtagt gtgtaatcgg gtgttgttta ttagcatatt atgttttggt 10921 cataggggaa attagaagtt gattacaagg ggttatgata tctatcctct ctagcttaag 10981 caaagcgtta gtttgaagag ctaaagttac atttatctgt gggaggagag gaggaataag 11041 ttaaacttaa aataaactgt gtggttcatg ttcacgtaat acggatatcg tttcctccta 11101 gtatgttagt tacacgttta aatgctattt tttctgcggt cagttcgttg gtcacggggg 11161 gatggggtga tagttattat cgttattgtt tgtttggttt attagtctct ttcttgctgt 11221 tgcggatacc ttatatatat ggaatgcagg ggtttgctct gtttattatt ttagtagtgt 11281 ttccattatt tatgtcctta ttattgtctc gggttgtaga tgtgggtcct acctcatttt 11341 ttagagggtt cgtgcccccc ggaactccct tgtggatagc tccttttgta tgcttggctg 11401 agactttgag gtatattgtc cggcccatag ttttgatgat tcgtcctttt gttaatttaa 11461 caattgggac tttgggggga gtagctatcg gcggcttagg ttttaagttt ggtgtatggg 11521 ttatctcctt tttgttcttg ttgttttttt atgaggtctt tgtggcgtta gtacattggt 11581 ttatagtctg taaaattcta tctttttctg aggatcacta ggtttggtcg atgcgagggt 11641 taattatcag cttatttggg attgtagggg tattttgttt ttcagtacta atgtttagtt 11701 ctaaaaattt atcctttttt tgattattct tggagttggc gacactttgt gctatacctt 11761 tattctttct aagaggggag gggggtgtgt tggtcggctt gtttaactat attgttattt 11821 ctagcatatc ttcttctttg atgctgtgtg ggatacttag tggggagctt ttggctcttt 11881 tggtggttgg tttgttgtta aagtttgggt tgttcccatt ctgggggtgg gtttataagg 11941 taggtctcgg gtctaattgg ttggttttgt gggttgtttc tacttttttg aagagtcccg 12001 tttttctttt ccccttcttt cttaggtgtg ggggttatag gttagtaaaa tatgtatgtt 12061 gtataacctt tttaggtctt tctgttttgt tttgattata tagcttaaat tgatatgctt 12121 gctgatgtca tatgatgttg tcttcaagcg cagcgttagt tgctatgtct ggtgtattag 12181 ctgcggatgt tttggctgct atcttttttg tatattgtgt ctggtgtagt ttggtggttg 12241 tttttttttc tttttatgag tatgattttc ttgggggtct gggttattat ttttggtttt 12301 gtttcttgtt aatttctatg ccggtttcta tttctatttt ttataagctg gtgatggctg 12361 tgggtatcta ttcttgctgg tttgtggttt tgtgttgttg ggttatttat agtgtttctg 12421 aacagtttta tttgttgaag tttgttatga gttttaggct tccaaaggcg tcgggttggt 12481 ttgtttcaga agtgtagttg ggtaacgtag tttaagttaa aattcctatc ttacacgtag 12541 gagatggatt tgtgttctgt tatcattttg gttgtgaaac tggggagggg gagacggcat 12601 agtttaagtt atagatacat gctctgcaag catgtggtgg agtttcctgc tgttggagag 12661 gcttgtttct agtttattta agaatgttgg cttgtcgtgt cagaggagta gaatttttac 12721 gaagcaaggt ggttttggtg ttgaattatg tttatttgat tattagaagc ttggtggcct 12781 ttgttattgt tatggtgttt gtggcttttt ttattttggg ggagcgtaag gtgttgggtt 12841 atatgcagat tcgtaagggt cctaaaaagg ttgggttatt tggtctgtta cagaggtttg 12901 ctgatttgtt gaagttggtt ataaagttta aggtgatgtt ttttcagaat cggacgtggt 12961 tgtcttgatt gggtgtgtat ttgttagttt ttttgtcgtg tggttattgt gttgttctgg 13021 cgctggcgta tagtgggatt agttctggtt atttgatgtt atgattttta gttttgacta 13081 gaataacagg ttatagatta ttgagggttg gttgggggtc tcataataag tttgctttgc 13141 taagatgtgt tcgttcggct tttggttctg tcagttttga ggcatgtttt atgtgtgttg 13201 tgataatggt ggcgattgtt gtggggactt acggtgttgc aagtttagtt acaagggctt 13261 ggttgattaa tttggttttg ccgttagttt atggcctgtg gttggttggt atactatgtg 13321 aatgtaatcg gactcctttg gattatgctg aggcggagag ggagttagtt aggggtttaa 13381 atactgaata ttgtaatgtt ccttttactt gtttattcgc ttgtgaatat ttgattatgg 13441 ttattttttc ttggttttct tctgtgttgt tttggggcgg gagtttaatt ataggtttga 13501 cgttatttca tattatgttt tttgtttggg ctcgtgctac tttgcctcgg gttcgttatg 13561 attattttgt aagatttatg tgacgttgcg ctattttagt gttggttttt tcgttttttt 13621 ttattattta ggtcgttggt ggtgcgtgta gattatggtg gtttgtagat cgtaaggctg 13681 ttaactttaa ggaggagttt tcccgcggac ggcgaacaat tagatagttt aagttagaat 13741 aatagctttg ggggttatag gtctcttata tttgagttaa ttgaatcgat agggctgctt 13801 agcaggttac tgtgatatag taattgtggg attttaatct tcgtcgatat gagggttctg 13861 agggtagctt aagggtttaa agctcaggat tcttactcct gcgatgtttg ggttatataa 13921 cctctcagga atgttgttag tgttttcttt gtgtattctg ttttttttgg ttttttttat 13981 ggttgttgtt tttcatctat ttgtgtgaaa tacagatttg ggtatatttt ctggggagcg 14041 gtcatgggtt aggtcttttg agtgtggttt tatagcgcag cgattggttg aaaaatattt 14101 tagttataca tattttattt tgttggtctt ttttgtggtt tttgatttgg aggtgtcttt 14161 attattaaat cttcccttgc aggggatttt atataagaat cttaggtatt atctattttt 14221 cttggttttg ttggcggttg gttttggtgt ggagattagt cgtgggtata ttcgttgaag 14281 gtattaggtg gggagttata gggtcgtcgc tgctaacgat gattagaata ttggtttatt 14341 cagcttcctt gttttattag caacctaggt tatgctgtac tgtttgtttt caaaacaaga 14401 ggagatttta gggtcggttg ctgaag // LOCUS Echinostomatidae_EPS4 14426 bp DNA circular 06-DEC-2018 DEFINITION Uncharacterized Echinostomatidae isolate PE4 mitochondrion, complete genome. ACCESSION Echinostomatidae_EPS4 VERSION KEYWORDS . SOURCE mitochondrion Uncharacterized Echinostomatidae ORGANISM Uncharacterized Echinostomatidae Unclassified. REFERENCE 1 (bases 1 to 14426) AUTHORS Adema,C.M. TITLE An uncharacterized physid snail from NM JOURNAL unpublished REFERENCE 2 (bases 1 to 14426) AUTHORS Robinson,A.J., Bennett,A.E., Britton,T.L., Buser,A., Campbell,M.L., Carroll,N.D., Chavez Trujillo,D.G., Cutillas,V., Das,P.K., Dugan,D.M., Graf,K.E., Jeshurin,M., Johnson,J.M., Locke,J.W., Marlenee,J.F., McCready,J.E., Medley,J.J., Potts,Z.L., Ruiz,R.E., Wright,K.T., Zlatkin,K., Bu,L., Natvig,D.O. and Adema,C.M. TITLE Direct Submission JOURNAL Submitted (06-DEC-2018) Biology, university of New Mexico, 2428 Castetter Hall University of New Mexico, Albuquerque, NM 87131, US COMMENT Bankit Comment: TAX: Yes, new species/combinations; SEE ATTACHMENT Bankit Comment: TOTAL # OF SEQS:1 Bankit Comment: SUBMITTER_TAX_REPLY: Yes, new species/combinations; parasite observed as echinostome-type cercaria, shed from physid snail, mitogenome sequence most similar to mitogenomes of echinostomatidae ##Assembly-Data-START## Assembly Method :: mitobim v. 1.8 Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..14426 /organism="Uncharacterized Echinostomatidae" /organelle="mitochondrion" /mol_type="genomic DNA" /isolate="PE4" /country="USA: Albuquerque NM" /lat_lon="35.2144 N 106.596 W" /collection_date="22-Jul-2016" /note="Lineage: Eukaryota; Metazoa; Platyhelminthes; Trematoda; Digenea; Plagiorchiida; Echinostomata; Echinostomatoidea; Echinostomatidae; echinostome-type parasite patent infection in physid snail" gene 1..1539 /gene="cox1" CDS 1..1539 /gene="cox1" /note="[intronless gene]" /codon_start=1 /transl_table=9 /product="cytochrome c oxidase subunit I" /translation="MYKWGWLFTLDHKRVGLIYMVIGLWGGFLGLSLSILIRLNYLDP YDNLVSPEIYNYVVTSHGIVMVFFFLMPVLIGGFGNYLLPILLGVPDLNLPRINALSA WLLLPAAVCLGLSMLGGAGVGWTFYPPLSTSTYSGWGVDFLMFSLHLAGVSSILGSIN FICTILEGLLEEGTGQFSIIIWAYLFTSILLLVSLPVLAAAITMLLFDRSFGSAFFDP LGGGDPVLFQHLFWFFGHPEVYVLILPGFGIISHICMTLTNNDSLFGYYGLILAMAAI VGLGSVVWAHHMFMVGLDVETAVFFSSVTMVIGIPTGIKVFSWLIMLGGTRGRLWDPV VWWIIGFIVLFTIGGVTGIMLSASILDTLLHDTWFVVAHFHYVLSLGSYSSVIISFIW WWPVITGYSLNYGMLWAHWGVSMVGFNLCFFPMHYLGMCGLPRRVCVYDPDLYWLNLC ASSGGIISGISAFFFVFILWESMLVRNRVASSWGSNSLVLNVVTLPTPHHMLYSVGGD RWLF*" tRNA 1570..1643 /product="tRNA-Thr" /anticodon=(pos:1609..1611,aa:Thr,seq:tgt) rRNA 1644..2620 /product="16S ribosomal RNA" tRNA 2630..2694 /product="tRNA-Cys" /anticodon=(pos:2660..2662,aa:Cys,seq:gca) Final sequences: >18S_EP1_18AAATGAACAAGTTTGGTCGTCNNNNCNGCNCCGGTCGAGCGGCCGAAGCCACTACAATGGCACCAATCCGAGGACCTTGCTAAACCATTCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAGCGCAAGCTTATGACTTGCACTTACCAGGAATTCCTCGTTCACGGGGAACAATTGCAAGCCCCGATCCCAGTCACGACGGTTATGATTCAGTTTACCCAACCTGCTCAGGCCAGGATTCCAAACTCGCTGAAACCGTCATTGTAGCGCACGTGCGGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATTTCGTGCGACTGGAGTCGCTTGTCCCTCTAAGAAGTAAGTACCTGCGCCGCACCCGCCGGTCAACGTAACCACTACCCCACAAGGAGGCAATGGACAACGTAACCTGCACGAGCACAGAGGACAGGCATACTATTTAGCAGGCCAAAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCAACCACCGAATCAAGAAAGAGCTATCAATCTGTCAATCCTCACAGTGTCCGGGCCGGGTGAGTTTTCCCGTGTTGAGTCGAATTGAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAGCCCAAAGACTTAAGGGTTTCCCGGGGACTGCCCGTGGGGACACATAAAGAACCACCACGGATCGTCAGTTGGCATCGTTTATGGTCAGAACTAGGACGGTANNNNNNNNTCTTCGAACCTCTGACTTTCGCTCAAGATCAATGAAAACATCCTTGGCAAATGCTTTCGCTGTAGTTTGTCTGGCGGCGATCCAAGAATTTCACCTCTAACACCGCCATACAAATGCCCCCGTCTGTCCCTCTTAACCATTACTTCGGATCCGAAAACCAACAAAATAGAACCGAAGTCCTATTCCATTATTCCATGCAAGAATTTTCAGGCACAAAGGCCTGCTTTGAGCACTCAAATTTGTTCAAAGTAAACGTGCTGTCCGCCTCCGACACCCGTTTAAAGGCATCAAGAAGCATGCCAACAGACCAGCAGGCAAGCCCACCAGTCACGCTGACACCTGTAAACAGACACAGCAGAAAGGAAGCACAACCACTAAACCGACTCGCAGGCTTGATCCGGTGGTTACCAGACCACACCAACGAGCGACKGCATGTAGCCATGCAACCCAGATCCAACTACGAGCTTTTTAACTGCAACAACTTTAATATACGCTTCTGGAGCTGGAGTTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAGTTGATCCTCGTTAAAGGATTTAAATTGTACTCATTCGAATTACGGAGCCTCTATTGAGTCCCGTATCCGTATTTTTCGTCACTACCTCCCCGTGCCGGGATTGGGTAATTTGCGTGCCTGCTGCCTTCCTTGGAAGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTAGAACCATGGTAGGCAGGTCACCTACCATCGTCAATTGATAGGGCAGACATTTGAAAGATCCGTCGCCGACACAAGGCCGACTGCGATCAGTGAAGTTATCCAGAGTCATCACAGAATGCAACAGGAACGACCGAAGCCGCTCCCGGTTGGTTCTGTTCTAATAAATCCACCCGTTCCCTTGCGGGTCAGGGCATAGTGGCATGTATTAGCTCTAGAATTACTACAGTTATCCATGTAGTATGTACGATCCAAGGAACCATAG SO: Genbank 16S PE1 MK237989 PE2 MK237990 LE5 MK237991 Ac2 MK237992 MT PES4 Physid MK242069 Echino MK264774
RETURN Lab coats Drawers Any liquids: sample layer buffer, EtOH, MQ Freezer boxes: DNA samples, LABELED! PE1, EP1 PE2, EP2 PE3, EP3 LE5, EL5 A1 (EP1), A2 (EP3) Anything else? Empty remainder into trash