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Innovative Microbubble Generation for Medical Applications by Shirley Zhang

Explore microfluidic devices creating double-layer bubbles containing lipid and oil surrounding gas. Test stability and attachment in vitro. Components include lipid, gas (PFC or N2), with a diameter of ~15 μm. Future directions involve using microbubbles for drug delivery in cell culture.

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Innovative Microbubble Generation for Medical Applications by Shirley Zhang

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  1. Microbubble Generation for Medical Applications Shirley Zhang Faculty Mentor: Abraham Lee Graduate Student: Kanaka Hettiarachchi

  2. Overview • Microfluidic devices for generating bubbles – test for optimal conditions • Create double layer bubbles to contain lipid and oil surrounding gas • Test stability and attachment of bubbles in vitro

  3. Making Devices U.V. Photoresist Mask Silicon wafer Develop Microfluidic channels PDMS

  4. Monolayer bubbles • Components: • Lipid (DSPE/DPPE-PEG2000) • Gas (PFC or N2) • Diameter: • ~15 um ColorCam 10x magnification

  5. Fluorescence • DiI mixed into lipids ColorCam 40x magnification

  6. Double Layer Bubbles • Components: • Lipid • Oil • Gas (PFC or N2) • No lipid shell – no concentrated fluorescence around bubbles FastCam

  7. Problems Triacetin DSPC/DSPE-PEG2K N2 FastCam

  8. Future Direction • Successfully make stable double layer bubbles • Microbubbles as a drug delivery system in cell culture

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