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Western Blotting of Respiratory Tract Proteins in HIV-positive Children

This study focuses on Western blotting of proteins isolated from bacteria and yeasts in the respiratory tract of HIV-positive children from Cambodia and Kenya. Monoclonal antibodies against HIV-1 gp120 were used. The results show the presence of HIV-like sequences in the bacterial and yeast samples. This hypothesis challenges the widely accepted theory of HIV transmission from apes to humans. The study also discusses the potential implications for research and treatment of AIDS.

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Western Blotting of Respiratory Tract Proteins in HIV-positive Children

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  1. Western blotting of proteins isolated from bacteria and yeasts of the respiratory tract (nose, pharyngeal swabs) of Cambodian (Km) and Kenyan (Ke) HIV positive children. Used monoclonal antibodies against HIV-1 gp120 (1:750). lines 1-6 tested samples: 14Km, 17’Ke, 21Ke, 32’Km, 3’Km, 31Km, 14’Ke line 7: serum of AIDS patient diluted 1:100; line 8: control bacteria; line 9: negative control bacteria Muta 104-0.

  2. Internalization of HL-60 cells by bacteria of AIDS patients and patientswith colorectal tumors(GPA) Patient/clone Number of bacterial colonies P15/7 2264 P1/4 1340 P3/3 1680 Mok12/5 lysis of HL-60 cells Mok 1/6 181 K1-1 lysis of HL-60 cells TuSG 71 883 S 104 negat. control < 5 ------------------------------------------------------------------------------------------ Number of HL-60 cells 2x106/ml Number of bact. cells 0,8x108

  3. Internalization of normal human lymphocytes by bacteria of AIDS patients (GPA) Patient/clone Number of bacterial colonies P15/7 1121 P1/4 complete lyses of human lymphocytes P3/3 complete lyses of human lymphocytes Mok12/5 complete lysis of human lymphocytes Mok 1/6 complete lysis of human lymphocytes K1-1 complete lysis of human lymphocytes Mok 22/5 423 725/5 1140 ------------------------------------------------------------------------------------------ Number of lymphocytes 2x106/ml Number of bact. cells 0,8x108

  4. Calculation of probiotic treatment (20 patients) Viral load in time of probiotics treatment start: 808 600 Viral load in term of the end of probiotic treatment: 452 190 Reduction of viral load after probiotic treatment: 356 410 (56%) Viral load of 11 patients from 20decreased (60%)

  5. The origin of HIV According to our and other results there is strong objectionagain generally accepted dogma that HIV was transmitted to humans from apesin Africa about 35-50 or according to last assertion 100 years ago en route of accidental contacts. There is not evidence about transmission of retroviruses between two different species in nature. Should be HIV only one exception in nature? Are there adequate proofs for this statement? The problem is that the acceptance of this dubious argument has a major influence on – research, diagnosis and therapy of AIDS. How is it possible that this unconvincing argument wasadopted by a ​​large part experts???

  6. Our hypothesis • HIV is of human origin and has been an integral part of humans from the beginning of our existance. We inherited it from our ancestors. Bacteria/yeasts are most likely natural host of HIV sequences. • But, there is serious objection – if HIV was in our body from our beginning, why did it’s emerge just about 25-35 years ago? To answer this question we should go back into the ancient history of humankind. In the past, major epidemics frequently occurred when there were new patterns of transport between separately populated areas.This tremendous longtime „sanitary“ process resulted in establishment of the balance between these two kingdoms. This balance was interrupted in the middle of the 20. century due to acceptation of ATBs, drugs and changes in life style (homo and anal sex). Propagated pathogenic microbes, some of which contain viral and HIV genetic information, are provided resistance to antibiotics and ability to penetrate into the body. When bacteria carrying the HIV-like sequences penetrated into the blood, interact with lymphocytes, infected/lyse them consequently the immune system has collapsed. The result of this process is .....AIDS

  7. This hypothese is assumed that transmission of HIV from apes to humans during last decades as a consequence of their accidental contacts - is not a cause of AIDS. The presence of HIV sequences in intestinal bacteria and yeasts of the patients may be explained as follows: 1) Based on our results, we assume that intestinal bacteria / yeast is a natural host of HIV sequences in extrachromosomal or other forms. 2) But it is conceivable that intestinal bacteria/yeast were infected with the HIV genetic material, previously present in human cells, particularly in macrophages / lymphocytes, after their degradation. • Our hypothesis may opens new opportunities for research and treatment of AIDS

  8. and now, we are able to answer on many until now unanswered questions

  9. Presented hypothesis answer to many until now unanswered questions: - origin of HIV - large scale HIV positivity in Africa, - connection of AIDS with TBC in Africa, - absence of „gold standard“ in Africa, - the presence of HIV reservoirs after antiretroviral therapy, - atypical course of disease in comparision with other retroviral infections - the rarity of complete viral particles detection in the material from AIDS patients, but detection of HIV sequences and the HIV-like proteins (antigens) herein - high AZT toxicity - and others.....

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