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Study of Mitosis in Garlic and Hyacinth Root Tips using Staining Techniques

This experiment involves observing mitosis in plant root tips using staining techniques with lactopropionic orcein and toluidine blue. The process includes staining, squash preparation, and calculating the mitotic index to analyze cell division.

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Study of Mitosis in Garlic and Hyacinth Root Tips using Staining Techniques

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  1. Cell division Staining a root tip to observe mitosis

  2. Plant material - Garlic - Hyacinth Stain - lactopropionic orcein - toluidine blue Mitosis

  3. Mitosis • Garlic clove • Polystyrene float

  4. Mitosis • Float in beaker of water and leave for two days

  5. Preheat 1 M HCl (~ 10 cm3) to 60°C

  6. Cut 2 mm from vigorously growing root tips • Add to pre-heated acid • Leave at 60°C for 4 – 5 mins

  7. Add large drop of water to slide

  8. Use a fine needle or forceps to transfer root tip from hot acid to drop of water on slide • Use paper towel to blot off as much water as possible

  9. Use a second slide to squash the root tip • This provides two root preparations

  10. Add a drop of stain – either toluidine blue or lactopropionic orcein

  11. Toluidine blue – Immediately cover with coverslip • Lactopropionic orcein - leave for 4 – 5 min, cover with coverslip

  12. Invert slide & blot firmly on wad of tissue • View preparation under microscope

  13. Mitotic index: Number of mitotic cells x 100 % Total number of cells

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