110 likes | 124 Views
Animal Breeding Techniques and Technologies. Artificial Insemination (AI). What is AI? Collecting semen from a male and artificially placing it directly into the uterus horn of a female. Teaser. Dummy. Electro ejaculator. Artificial Insemination (AI). Artificial Insemination (AI).
E N D
What is AI? Collecting semen from a male and artificially placing it directly into the uterus horn of a female Teaser Dummy Electro ejaculator Artificial Insemination (AI)
Artificial Insemination (AI) • Why use AI? • To make widespread use of males with • high genetic merit • Cost beneficial • E.g. stud ram cost $6000/100 ewes = $60 per head mated • total AI cost $3000/100 ewes = $30 per head mated • National access to superior males • Disadvantages • Time consuming, requires planning • Technical expertise, errors can be costly
AI Methods • Laparoscopic Technique • Place semen directly into uterus horn through the abdominal cavity • Cervical Technique • Place semen in uterus horn directly through the cervix
Selecting Sires and Dams • The sire (male) will influence rate of genetic gain more strongly than the dam (female) • Sires (semen) • High genetic merit • Semen quality and quantity • Dams • Genetic merit, mothering traits • Body condition
Starting AI Programme • Animals selected for AI should be in good health, body condition score ideally 3 - 3.5 • Day 1: A CIDR containing progesterone is inserted intravaginally. This suppresses oestrus and ovulation in the ewe. • Day 13: CIDR is removed. Ewe is injected with PMSG which stimulates ovarian follicle development (oestrus) • 48-52 hours later: AI surgery CIDR: controlled internal drug release PMSG: pregnant mare serum gonadotrophin
AI Technique • Animals are fasted for 12-24 hours • Animal is prepared for surgery: sedated, put in cradle, wool clipped, site sterilized, local anesthetic • Semen straw is brought to body temperature • Abdomen is punctured with trocar, CO2 is pumped into the cavity to make the area more accessible. • A laparoscope (light source) is inserted down the trocar cannula • On opposite side forceps are inserted to hold the uterus horn • Semen straw is inserted through the abdomen, and semen is injected into the uterus horn • Topical antibiotic powder applied.