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What is Bio-technology? Why is it important to understand your genetic make up? How does this information affect your life? Society?. http://www.komonews.com/news/consumer/17071161.html.

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komonews/news/consumer/17071161.html

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  1. What is Bio-technology?Why is it important to understand your genetic make up?How does this information affect your life? Society? http://www.komonews.com/news/consumer/17071161.html Advancement of technology for research associated with life science. Especially Genetic Engineering (changing genes and recombinant DNA (changing DNA sequences)

  2. Eliminating crime at EHS by incorporating electrophoresis into our discipline policy. Would you like to help? • Tools you need to learn to use… Micro pipettes… Electrophoresis… Before we can use these tools we will need training on how they work…

  3. What is the metric system?. Standard measurement system used through out the world • How big is a Liter? • How big is a mili Liter? • mL(1/1000 L) mili means one thousanth • How big is a micro liter compared to a L and a mL? • (1/1000 of a mL) (1/1,000,000 of a L) • Which of these units do you think would be the best for looking at pieces of DNA? • Why?

  4. The MICRO LITER!! • DNA IS SO SMALL THAT IT NEEDS TO BE LOOKED AT IN VERY TINY PIECES • What tool should we use to measure DNA? • Micro-pipets allow us to take samples as small as .5 µL and as large as 1000 µL (1 mL)

  5. Are you a metric system master? • When I convert from L to mL will the number get smaller or larger? • Larger, I will move my decimal place 3 spaces to the right because it is a thousand times smaller. • When I convert from mL to µL will the number get smaller or larger? • Larger, I will move my decimal place 3 places to the right.

  6. Metric System Master? • When I convert from µL to mL will the number get smaller or larger? • Smaller, I will move my decimal place 3 places to the left. For example 2 µL = .002 mL • When I convert from mL to L will the number get smaller or larger? • Smaller. I will move my decimal place 3 spaces to the left. • 2.0 mL = .002 L

  7. Factor Label Method • A great way to set up conversions • Start the conversion with what you know and make a relationship that relates to what you want to know • For example when determining how many µL are in 20 L set up this conversion 20L 1,000,000 µL 1 L This means 20 X 1,000,000 =20,000,000 µL

  8. Metric System Master??? • If I have L and want µL what should I do? • Move the decimal place 6 places to the right • Or set a conversion using factor label method

  9. I will give you a measurement and you must tell me how many µL I have… Use Factor Label method • 2 µL = how many L? • 2 µL 1 L 1,000,000 µL 2µL X 1L /1,000,000µL = • .000002L • 87 mL = how many µL? 87mL 1,000 µL 1 mL 87mLx 1000 µL/ 1mL • 87,000 µL

  10. Try these on your own…. • 100 µL = How many L • 2,300,000 L = How many mL • 45 L = How many µL? • .0001 • 2,300,000,000mL • 45,000,000 µL

  11. EQUIPMENT!!! • You must show DeNA locator competentence before you can join the EHS crime fighting team!! • P-10 range of .5-10 µL • P-20 range of 2- 20 µL • P-200 range of 20-200 µL • P 1000 range of 200-1000 µL

  12. IF YOU USE THE WRONG PIPETTE IT WILL MAKE THEM LESS ACCURATE AND MAY DESTROY THEM..AND THEY MIGHT EXPLODE! • If I want to measure 1.2 µL which pipette would I choose? • P-10 • If I want to measure 178 µL which pipette would I choose? • P-200 • If I want to measure 778 µL which pipette would I choose? • P-1000

  13. Try these on your own. • Tell me the pipet for the specific measurement. • 600 µL 25 µL • 225 µL 90 µL • 1 µL 18 µL • P-1000 4. p-200 • P-1000 5. P-200 • P-10 6. P-20

  14. Use Factor Label to solve the following How many micro Liters are in 27 L? How many mL are in 23.4 micro Liters? How many micro Liters are in .234 L?

  15. Measure for Measure: You need Pipettes- May have to share p-10 and p-1000 Set of Tips for p-20/p-200 and p-10’s will share p-1000 Parafilm Microtubes (3) Sample of water

  16. How could we determine the components of a mystery dye mixture using electrophoreis?

  17. Purpose: To determine the dyes in two mystery mixtures: XXX and ZZZ

  18. Procedure: Materials micropipet tips micropipets blot paper (1 per person) ziplock bag acetate sheets (1 per person) plastic wrap marker pen ruler (mm) microtube rack microtubes • electrophoresis apparatus • (BRL Horizon 58 gel box) • Power Supply • (shared by two groups) • beaker for used tips • 1% agarose gel • 1X TAE buffer (enough to cover gel) • 6 known dyes • 2 mystery mixtures (XXX and ZZZ)

  19. Steps See Handout:

  20. Data: Distance traveled (in mm) To which pole +/- Comments & Observations Picture of Gel Before and After

  21. For Tommorrow: Finish Stuff to know before you Go: In reading notebook Pre-Lab- Purpose,Procedure,Data Section

  22. How should I take data? Look at get on light box Record DNA distances on acetate Compare # of bands and distance of bands

  23. Work on Conclusion after you’re done! If you will not be here Monday/Tuesday or Wednesday you must keep up! We will quiz Monday/Tuesday after break as well as have a GATTACA discussion

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