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Particle Measurement & Validation bv

Particle Measurement & Validation bv. Erkenningsnummer 3445-00. Peter van ’t Erve Project Manager International Service. Particle Measurement & Validation bv. Erkenningsnummer 3445-00. Introduction Basic stuff HEPA-filtration Types of cabinets Maintenance Criteria Disinfection

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Particle Measurement & Validation bv

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  1. Particle Measurement& Validation bv Erkenningsnummer 3445-00 • Peter van ’t Erve • Project Manager • International Service

  2. Particle Measurement & Validation bv Erkenningsnummer 3445-00 • Introduction • Basic stuff • HEPA-filtration • Types of cabinets • Maintenance • Criteria • Disinfection • HEPA-filter change • Miscellaneous

  3. Basic Stuff

  4. Basic stuff Basically a cabinet consists of : • The Body : Stainless Steel Epoxy coated steel • Prefilter : Eff. 80 – 90% acc. EN 779 • HEPA-filters : Eff. 99,99% acc. EN 12469 • Fan : To create the velocity • Electronics : To control the velocity and the alarm features • Accessories : on customer requirement

  5. Basic stuff Pre-filters Filter classification according EN 779 Filter type Pre-filters & Fine-filters Filter class G1 G2 G3 G4 F5 F6 F7 F8 F9 Am % >65 65<80 80<90 90 - - - - - Em % - - - - 40<60 60<80 80<90 90<95 95 • Cheap glassfiber filter-material, to protect the expensive HEPA-filters • To be changed every year • Low cost exchange • Lifetime of a HEPA: without Prefilter appr. 6 years with Prefilter : appr. 12 years

  6. Basic stuff H.E.P.A. filters • HEPA = High Efficiency Particulate Air • ULPA = Ultra Low Particulate Air The purpose of a HEPA-filter is to catch small particles. Construction of a HEPA-filter: • Filter media (Glass fiber or similar) The media is pleated to a package of a certain size. The pleated media is glued together in a frame

  7. HEPA-Filtration

  8. HEPA-Filtration: Efficiency • The efficiency of a HEPA-filter according EN 12469: 99,99% when tested by means of a photometer. 100.000 particles 10 Particles

  9. HEPA-Filtration Sieve operation of a HEPA

  10. HEPA-Filtration Interception/impaction

  11. Types of cabinets

  12. Product Protection • Personal protection • Product and personal protection Types of cabinets: General

  13. Crossflow • Downflow unit • Laminar Downflow Types of cabinets: Product protection

  14. Class II B Class II A Class II A (Double exhaust) HEPA-Filter Pre-filter Types of cabinets: Biological safety cabinets

  15. Inflow Recirculation Pressure plenum Downflow Exhaust Types of cabinets: Biological safety cabinets Flow pattern 30% 70%

  16. Maintenance

  17. Maintenance: General • Check on Pre-filter • Airflow velocity’s downflow & inflow • Filter efficiency test by means of a photometer or particle counter • Check on alarmsettings

  18. Product Protection • Personal protection • Product and personal protection Maintenance: Control of Pre-filter When do we need to change it? Turn over one edge of the pre-filter, If the down side is becoming grey, replace it. Take care of contaminated areas !!

  19. Maintenance: Controlof air flow and velocity

  20. Maintenance: Airflow measurements (EN 12469) Airflow velocity measurements • Downflow • Measurements have to be taken at • - 8 positions minimum, • 50 to 100 mm above the top edge of the front aperture. • Criteria: • All values have to be >0,25 and < 0,50m/s; • According manufacturer’s specifications; • No individual measurement should deviate more than 20% from the mean.

  21. 1 2 3 Window a a Working space H b c b c L 1/8 L 4/8 L 7/8 L Front view Side view Maintenance: Airflow measurements (EN 12469) Airflow velocity measurements Inflow According Annex G you have to measure the velocity in the exhaust duct and calculate the volumetric rate of discharged exhaust air. With this air volume you can calculate the mean inflow velocity. In practice it is much more easier to measure the inflow velocity in the front aperture. Criteria: Mean inflow velocity > 0,40 m/s and, according manufacturer’s specifications.

  22. Maintenance: Airflow measurements (EN 12469) Flow pattern Tested using smoke Inflow: The direction of the inflow should be inward over the whole area of the front aperture. Downflow: The direction of the flow should be downwards without turbulence as a result of options or other obstructions in the working area.

  23. Maintenance: Control ofHEPA filter efficiency Procedure: Option 1. Filtertest with a Particle Counter and a test aërosol. Option 2. Efficiency test with a Photometer and a test aerosol. • Criteria: • Tested with a Photometer a local penetration should be not more than 0,01%. • Related Standards: • IES-RP-CC006.2 • ISO/CD 14644-3 • EUROVENT 4/8

  24. Generating the test aerosol (DOP, Emery 3004 , DEHS) Referential measurement (80-120 µgr / L) 100% measurement Scanning probe Photometer Maintenance: Control ofHEPA filter efficiency Downflow / Crossflow unit Do not take a sample of the HEPA-filter !!!!!!!

  25. _ + Generating the test aerosol (DOP, Emery 3004 , DEHS) 100% + _ + + + + 100% measurement (80-120 µgr / L) _ + + + Probe Scanningprobe _ Photometer _ _ _ Maintenance: Control ofHEPA filter efficiency

  26. Generating the test aerosol (DOP, Emery 3004 , DEHS) 100% measurement (80-120 µgr / L) Scanningprobe Photometer Probe 100% Maintenance: Control ofHEPA filter efficiency

  27. Maintenance: Alarm Settings (EN 12469) Inflow • High Alarm • Create a high alarm by increasing the voltage of the fan and check whether there is a high alarm. • Low Alarm • Create a low alarm by de-creasing the voltage of the fan and check whether there is a high alarm. Downflow • High Alarm • Create a high alarm by increasing the voltage of the fan and check whether there is a high alarm. • Low Alarm • Create a low alarm by de-creasing the voltage of the fan and check whether there is a high alarm. Window Alarm (If applicable) Check whether there is an alarm if the window is not in a calibrated position. Other alarmpoints • Check all other alarmpoints

  28. Maintenance: Setting the alarms • Adjust velocity • Determine High alarm • (Vav + 20%) High alarm • Determine Low alarm • (Vav - 20%) Vav Low alarm • Adjust alarmwindow to calculated alarmsettings • Adjust alarmwindow to calculated alarmsettings • First High alarm • Adjust alarmwindow to calculated alarmsettings • First High alarm • Then Low alarm • Make your alarm • window as big as possible

  29. Maintenance: Testpoints CA(B) / x and EF(B) / x cabinets A: Aerosol supply B: 100% measuring point (and test point for overall efficiency test 1st HEPA-filters on a CB and EFB) This point might also be on top of the cabinet for a CA(B) C: Scanning downflow HEPA-filter D: Scanning exhaust HEPA-filter (overall efficiency test on a CA(B)

  30. Ducting: Ways of ducting There are three ways to connect a safety cabinet to a duct! • Direct connection! • Direct connection with a by-pass! • Connection via a thimble!

  31. Externalfan Anti Blow Back Valve Airsupply Ducting: Direct connection • Both cabinet and the external ventilator have to be switched on at the same time, this is done by a potential free contact (optional accessory) coming from the cabinet! • The anti blow-back-valve assures that no contaminated air can travel back into the cabinet! • The room pressure will fluctuate depending if the cabinet is switched on or off. However it is possible to have the air inflow adjusted to the amount of air that is extracted from the room! • Advantage: Higher level of safety!

  32. Externalfan Anti Blow Back Valve By-pass valve Airsupply Ducting: Bypass connection • The external ventilator works 24hrs a day! • When the cabinet is switched on the by-pass valve in the duct will open in order to extract the exhausted air from the cabinet. • When the cabinet is switched off the valve will close and will extract the same amount of air from the room! • The valve is also controlled by a potential free contact coming from the cabinet. • The anti blow-back-valve assures that no contaminated air can travel back into the cabinet! • Advantage: Higher level of safety and the room pressure will be constant at all time!

  33. Externalfan Airsupply Ducting: Via a Thimble • Effects on the roompressure: • When the cabinet is switched on the exhausted air will be extracted from the cabinet! • When the cabinet is switched off the exhausted air will be extracted from the room! • Advantage: No adjustments have to be made to the cabinet and the pressure in the room will be constant!

  34. What will be the effect on the alarm settings if the cabinet is connected on an exhaust duct work? There are three possibilities: • There is no extra fan in the exhaust system. Adjusting the alarms can be done by increasing or decreasing the downflow and inflow air velocity by means of the fan of the cabinet. • There is an extra fan in the exhaust system, but there is no link between the status (Day- or Stand-by position) of the cabinet and extra exhaust fan. • There is an extra fan in the exhaust system, with a link between the status (Day- or Stand-by position) of the cabinet and extra exhaust fan.

  35. Disinfection

  36. HEPA-filtered air HEPA-filtered air mixed with product Air mixed with fresh air and product Contaminated air Disinfection • Why desinfection: - Microbiological Safety Cabinet is a Biohazard. - All parts in the cabinet are a possible risk for both personnel and environment when opened. • When desinfection: - If repair needs to be done inside the cabinet. • With what can we desinfect a cabinet - That depends on the product which was handled in the cabinet, responsible for desinfection is the head of the laboratory.

  37. Clean Air techniek offers a so called FAS 2000 which is using Formalin for desinfection and Ammonia to neutralize the Formalin afterwards. HEPA-filtered air HEPA-filtered air mixed with product Air mixed with fresh air and product Contaminated air FAS 2000 Disinfection Using Formalin

  38. Depending on the size (volume) of the cabinet, Formalin is put in the can with the red cap and Ammonia is put in the can with the blue cap. FAS 2000 Disinfection Using Formalin

  39. In order to prevent Formaldehyde of getting into the laboratory, the cabinet has to be sealed off. • You can do this by sealing the cabinet with a plastic bag. Make sure that the bag is sealed to the floor. Disinfection Using Formalin

  40. HEPA-filter change

  41. HEPA-filter change 1st HEPA • First HEPA-filter on a Class IIB cabinet:

  42. HEPA-filter change 1st HEPA • Take of the pressurebar. (On older models you have to cut the silicon along the outer edge of the filter.)

  43. HEPA-filter change 1st HEPA • Seal the opening of the HEPA-filter in order to prevent parts to come out of the HEPA-filter

  44. HEPA-filter change 1st HEPA • Replace the HEPA by a new one and put the pressure bar back in it’s place.

  45. Take of the backpanel • Disconnect the fan and take it out HEPA-filter change Downflow HEPA

  46. Unloosen the pressure bars of the downflow plenum and the exhaust HEPA and take them out. • Lift the pressure plenum and take it out. • Take out the HEPA-filter. Pressure bars Plenum HEPA-filter HEPA-filter change Downflow HEPA

  47. Put in a new HEPA-filter with the seal downwards and put everything back in it’s place. HEPA-filter change Downflow HEPA

  48. Lift the exhaustduct • Unloosen the pressurebars and take them out • Lift the pressure plenum and take it out • Take out the HEPA-filter • Put in a new HEPA-filter with an extra seal on one end. • Put everything back in it’s place HEPA-filter change Exhaust HEPA

  49. Criteria Criteria

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