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The regular mechanism of eukaryotic DNA replication

The regular mechanism of eukaryotic DNA replication. Abstract ： 1 、 conserved initiator,divergent replicator 2 、 the origin recognition system 3 、 replication licensing system 4 、 SPF 3th group ：罗通 郭鹏 杨孔. Conserved intiator,divergent replicator.

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The regular mechanism of eukaryotic DNA replication

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  1. The regular mechanism of eukaryotic DNA replication • Abstract： 1、conserved initiator,divergent replicator 2、the origin recognition system 3、replication licensing system 4、SPF 3th group：罗通 郭鹏 杨孔

  2. Conserved intiator,divergent replicator

  3. Xenopus eggs and egg extracts • Xenopus eggs and egg extracts • Three distinct system: the origin recognition system（ORC） the replication licensing system S phase-promoting factor ,SPF （Cdc7,CDKs)

  4. the origin recognition system（ORC） • In the early Xenopus embryo, replication origins are positioned randomly with respect to DNA sequence • then,origins become restricted to specific DNA seqences. • It seem likely that The periodic spacing of replication origins is mediated by periodic binding of ORC along the chromosomal DNA. • Two different model of periodic spacing of ORC.

  5. replication licensing system • 复制认证系统的组成： Fig.4认证模式的必需组件。起点的认证通过 起点结合Mcm(2-7)蛋白实现的。Mcm(2-7)是必须的，但其功能不清楚。可能是起解旋酶的作用。 • 认证系统的启动时间和抑制时间：Cdt1、Cdc6结合到起点认证开始，S期开始前受到抑制。 • 认证系统的调控和作用。Fig-5。 认证系统的沉默受到多重调节（1、2、3、RLF-B/Cdt1抑制子，S、G2期积累，中后期转换时突然降解，此时认证系统被启动）。

  6. Fig、4

  7. Fig、5

  8. 四、SPF • SPF的组成：Cdc7和CDK s（周期依赖蛋 白激酶）。 Cdc7在减数分裂末到S期末召募到已被认证的染色体上。 CDKs:B,A, E . • SPF的调控和作用：不依赖周期蛋白的丰富程度和核的超级结构，可能是由于Cdk-cyclin E功能的全部启动依赖与 Cdc34 介导的蛋白水解作用，这种水解作用只能在核质中启动。 • SPF的抑制时间:在减数分裂晚期和G1期受到抑制。

  9. thanks

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