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A. B. C. D. Supplementary Figure S3. Edman degradation and mass spectrometry analysis of 32 P phosphorylated FOXO3a peptides

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  1. A B C D Supplementary Figure S3. Edman degradation and mass spectrometry analysis of 32P phosphorylated FOXO3a peptides A. Peak 1 contained a peptide corresponding to residues 291-298 of FOXO3a phosphorylated at S294. B. Peak 2 contained a peptide corresponding to residues 420-444 of FOXO3a phosphorylated at S425. C. Peak 3 contained a peptide corresponding to residues 324-368 of FOXO3a phosphorylated at S344. D. Peak 4 contained a peptide corresponding to the 12 C-terminal residues of the GST tag and residues 1-27 of FOXO3a phosphorylated at S12. The apparent release of 32P at cycle 17 in peak 3 can be explained by the coupling of a proportion of the peptide to the solid phase support solely through the side-chain carboxyl group of the aspartic acid residue.

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