F3c

1. B2 Primer annealing FIP F3 LBc F2c B1 B2 B3 LF F3c F1c F2 B1c B2c F3 F1 LB B3c LFc A B3 BIP Stem-loop structure and loop primers LB F2c F1c B1 LF B2c F1 B1c B1c F2 F1 FIP BIP F1c B1 B Inverted repeats concatamers C Supplemental Data Figure 1. LAMP amplification mechanism Scheme of the amplification mechanism of LAMP reaction. F3, B3, FIP and BIP primers anneal on the target in the showed positions (A) and by the peculiar amplification and displacement mechanism they are responsible for the formation of the “dumb-bell” starting structure, from which the whole reaction starts in an exponential-like fashion. Additional loop primers (LF and LB) allow an increase in the amplification rates by annealing on single strand regions of the loops in the dumb-bell structure (B). Concatamers made by repetitions of the initial module are produced (together with other structures) during the reaction